| Literature DB >> 20882320 |
Yongxin Yang1, Jiaqi Wang, Dengpan Bu, Leying Zhang, Shanshan Li, Lingyun Zhou, Hongyang Wei.
Abstract
A sensitive and convenient "visible SYPRO" staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO Ruby or Blue Silver, respectively. In addition, this method was more sensitive than that of Blue Silver, simpler than that of SYPRO Ruby, and compatible with subsequent mass spectrometry analysis.Entities:
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Year: 2010 PMID： 20882320 DOI： 10.1007/s10529-010-0404-8
Source DB: PubMed Journal: Biotechnol Lett ISSN： 0141-5492 Impact factor: 2.461