BACKGROUND: Myelin-associated molecules are major impediments to axon regeneration after optic nerve injury. Intravitreal injection of α-crystallin can protect axons from optic nerve degeneration after crushing in rats. OBJECTIVES: Our purpose was to investigate whether α-crystallin could counteract the inhibitory effect of myelin and promote neurite growth. METHODS: Newborn rat retinal neurons were cultured on myelin-coated dishes with DMEM containing α-crystallin (10(-4) g/l) or bovine serum albumin. The density of neurons with neurites and the length of the longest neurite of the cells were analyzed on days 1, 3 and 5. RESULTS: Cultures containing α-crystallin had significantly higher neurite-containing cell densities, and the neurites were significantly longer compared with cultures containing bovine serum albumin. These findings indicated that α-crystallin could counteract the effect of myelin inhibitory factors and stimulate neurite growth.
BACKGROUND: Myelin-associated molecules are major impediments to axon regeneration after optic nerve injury. Intravitreal injection of α-crystallin can protect axons from optic nerve degeneration after crushing in rats. OBJECTIVES: Our purpose was to investigate whether α-crystallin could counteract the inhibitory effect of myelin and promote neurite growth. METHODS: Newborn rat retinal neurons were cultured on myelin-coated dishes with DMEM containing α-crystallin (10(-4) g/l) or bovineserum albumin. The density of neurons with neurites and the length of the longest neurite of the cells were analyzed on days 1, 3 and 5. RESULTS: Cultures containing α-crystallin had significantly higher neurite-containing cell densities, and the neurites were significantly longer compared with cultures containing bovineserum albumin. These findings indicated that α-crystallin could counteract the effect of myelin inhibitory factors and stimulate neurite growth.
Authors: Joseph C Boktor; Mark D Adame; Destanie R Rose; Cynthia M Schumann; Karl D Murray; Melissa D Bauman; Milo Careaga; Sarkis K Mazmanian; Paul Ashwood; Brittany D Needham Journal: Mol Psychiatry Date: 2022-08-26 Impact factor: 13.437
Authors: Johannes M van Noort; Malika Bsibsi; Peter J Nacken; Richard Verbeek; Edna H G Venneker Journal: PLoS One Date: 2015-11-23 Impact factor: 3.240