A D Adekile1, M Z Haider. 1. Department of Paediatrics, Faculty of Medicine, Health Sciences Centre, Kuwait University, Kuwait. adekile@hsc.edu.kw
Abstract
OBJECTIVE: To investigate the prevalence of haptoglobin (Hp) gene alleles in Kuwaiti sickle cell disease (SCD) patients, who generally have a mild phenotype, and compare the pattern to Nigerian SCD patients whose SCD phenotype is more severe. SUBJECTS AND METHODS: Hp genotyping was carried out in a group of 82 and 54 SCD patients from Kuwait and Nigeria, respectively, and appropriate Hb AA controls. The Hp genotyping was done using a PCR technique followed by agarose gel electrophoresis. RESULTS: The frequency of the Hp-2 allele was 73.8% among Kuwaiti SCD patients, while the Hp-1 allele predominated among Nigerian patients (60.7%). However, the differences were not significant (p > 0.05) when the allele distributions were compared between Kuwaiti SCD and their AA counterparts or between Nigerian SCD and their AA controls. There was no association of Hp-2 allele with frequent vaso-occlusive crisis among the Kuwaiti SCD patients. CONCLUSION: The distribution of Hp alleles appears to follow ethnic and geographical trends. Their role in the pathophysiology of pain crisis is not clear.
OBJECTIVE: To investigate the prevalence of haptoglobin (Hp) gene alleles in Kuwaiti sickle cell disease (SCD) patients, who generally have a mild phenotype, and compare the pattern to Nigerian SCDpatients whose SCD phenotype is more severe. SUBJECTS AND METHODS: Hp genotyping was carried out in a group of 82 and 54 SCDpatients from Kuwait and Nigeria, respectively, and appropriate Hb AA controls. The Hp genotyping was done using a PCR technique followed by agarose gel electrophoresis. RESULTS: The frequency of the Hp-2 allele was 73.8% among Kuwaiti SCDpatients, while the Hp-1 allele predominated among Nigerian patients (60.7%). However, the differences were not significant (p > 0.05) when the allele distributions were compared between Kuwaiti SCD and their AA counterparts or between Nigerian SCD and their AA controls. There was no association of Hp-2 allele with frequent vaso-occlusive crisis among the Kuwaiti SCDpatients. CONCLUSION: The distribution of Hp alleles appears to follow ethnic and geographical trends. Their role in the pathophysiology of pain crisis is not clear.
Authors: Shaina M Willen; Joel Brennan McNeil; Mark Rodeghier; Vern Eric Kerchberger; Ciara M Shaver; Julie A Bastarache; Martin H Steinberg; Michael R DeBaun; Lorraine B Ware Journal: Am J Hematol Date: 2020-01-30 Impact factor: 13.265
Authors: Sameer Al Zenki; Husam Alomirah; Suad Al Hooti; Nawal Al Hamad; Robert T Jackson; Aravinda Rao; Nasser Al Jahmah; Ina'am Al Obaid; Jameela Al Ghanim; Mona Al Somaie; Sahar Zaghloul; Amani Al Othman Journal: Int J Environ Res Public Health Date: 2015-07-31 Impact factor: 3.390
Authors: Christian Bernard Kengne Fotsing; Constant Anatole Pieme; Prosper Cabral Biapa Nya; Jean Paul Chedjou; Solange Dabou; Carine Nguemeni; Georges Teto; Wilfred Fon Mbacham; Donatien Gatsing Journal: Health Sci Rep Date: 2022-01-21