PURPOSE: To assess a rat model of cerebral ischemia induced by occlusion of the middle cerebral artery and its effect on the area of cerebral infarction. METHODS: Brain ischemia was induced in 52 male Wistar rats by introduction of a 3-0 nylon suture into the middle cerebral artery for either 90 (n=28) or 120 (n=24) minutes. Ischemic injury volume was determined by TTC staining, digital photography and analysis with the Image J software. Statistical analysis employed Student's t test and the Mann-Whitney U test. RESULTS: The groups were similar in terms of weight (p=0.59). The length of thread inserted was 14.7 mm in the 90 min group and 20.2 mm in the 120 min group (p=0.37). Ischemic injury was detected in 11 animals (39%) after 90 min and 11 (45%) after 120 min (p=0.77). In animals exhibiting injury, filament length was 16.1 ± 11 mm (90 min) vs. 21.9 ± 7.4 mm (120 min) (p=0.15). The mean infarction zone volume was greater after 120 (259.2 mm³) than after 90 min (162.9 mm³) (p=0.04). The neurological deficit score for the 90 and 120 min groups was 2.0 and 2.4, respectively (p=0.84). CONCLUSION: The experimental model induced significant ischemic cerebral injury in both groups.
PURPOSE: To assess a rat model of cerebral ischemia induced by occlusion of the middle cerebral artery and its effect on the area of cerebral infarction. METHODS:Brain ischemia was induced in 52 male Wistar rats by introduction of a 3-0 nylon suture into the middle cerebral artery for either 90 (n=28) or 120 (n=24) minutes. Ischemic injury volume was determined by TTC staining, digital photography and analysis with the Image J software. Statistical analysis employed Student's t test and the Mann-Whitney U test. RESULTS: The groups were similar in terms of weight (p=0.59). The length of thread inserted was 14.7 mm in the 90 min group and 20.2 mm in the 120 min group (p=0.37). Ischemic injury was detected in 11 animals (39%) after 90 min and 11 (45%) after 120 min (p=0.77). In animals exhibiting injury, filament length was 16.1 ± 11 mm (90 min) vs. 21.9 ± 7.4 mm (120 min) (p=0.15). The mean infarction zone volume was greater after 120 (259.2 mm³) than after 90 min (162.9 mm³) (p=0.04). The neurological deficit score for the 90 and 120 min groups was 2.0 and 2.4, respectively (p=0.84). CONCLUSION: The experimental model induced significant ischemic cerebral injury in both groups.
Authors: Ji Heun Jeong; Kwang Sik Yu; Dong Ho Bak; Je Hun Lee; Nam Seob Lee; Young Gil Jeong; Dong Kwan Kim; Jwa-Jin Kim; Seung-Yun Han Journal: Exp Ther Med Date: 2016-10-31 Impact factor: 2.447
Authors: Yuriy I Sysoev; Veronika A Prikhodko; Aleksandra V Kan; Irina A Titovich; Vadim E Karev; Sergey V Okovityi Journal: Neurol Int Date: 2022-06-21
Authors: Ji Heun Jeong; Shin Hye Kim; Mi Na Park; Jong Yea Park; Hyun Young Park; Chan Eui Song; Ji Hyun Moon; Ah La Choi; Ki Duck Kim; Nam Seob Lee; Young Gil Jeong; Do Kyung Kim; Bong Ho Lee; Yung Choon Yoo; Seung Yun Han Journal: Curr Issues Mol Biol Date: 2021-06-15 Impact factor: 2.976