PURPOSE: Quantify the levels of oxidative DNA damage of epithelial colon cells comparing segments with and without fecal stream. METHODS: Sixty Wistar rats were subjected to deviation of fecal stream by proximal colostomy and a distal mucosal fistula. Animals were divided into three experimental groups that were sacrificed 6, 12 and 24 weeks after surgery. In each experimental group, five animals underwent laparotomy without intestinal deviation (sham subgroup). The diagnosis of colitis was made by histopathological analysis and the inflammatory activity index by graduated scale. The neutrophil infiltration was determined by myeloperoxidase tissue levels and the intensity of oxidative DNA damage by comet assay. The Mann-Withney and Student t test were used to compare the results among experimental subgroups and the Kruskal-Wallis test for variance analysis, adopting a significance level of 5% (p<0.05). RESULTS: Colon segments without fecal stream was shown higher histological inflammatory score of the colon wall after 12 and 24 weeks (p=0.001) that increased with the time of diversion (p=0.01). The activity of myeloperoxidase in segments without fecal stream decreased with the time (p=0.001). Oxidative DNA damage levels were significantly higher in the segments without fecal stream, (p=0.0001), independent of time of colon diversion, and increase with the time (p=0.0007). CONCLUSIONS: Colon segments without fecal stream showed high levels of oxidative DNA damage related to histological alterations observed in diversion colitis. The levels of oxidative DNA damage in segments devoid of the fecal stream increase with the time of intestinal exclusion.
PURPOSE: Quantify the levels of oxidative DNA damage of epithelial colon cells comparing segments with and without fecal stream. METHODS: Sixty Wistar rats were subjected to deviation of fecal stream by proximal colostomy and a distal mucosal fistula. Animals were divided into three experimental groups that were sacrificed 6, 12 and 24 weeks after surgery. In each experimental group, five animals underwent laparotomy without intestinal deviation (sham subgroup). The diagnosis of colitis was made by histopathological analysis and the inflammatory activity index by graduated scale. The neutrophil infiltration was determined by myeloperoxidase tissue levels and the intensity of oxidative DNA damage by comet assay. The Mann-Withney and Student t test were used to compare the results among experimental subgroups and the Kruskal-Wallis test for variance analysis, adopting a significance level of 5% (p<0.05). RESULTS: Colon segments without fecal stream was shown higher histological inflammatory score of the colon wall after 12 and 24 weeks (p=0.001) that increased with the time of diversion (p=0.01). The activity of myeloperoxidase in segments without fecal stream decreased with the time (p=0.001). Oxidative DNA damage levels were significantly higher in the segments without fecal stream, (p=0.0001), independent of time of colon diversion, and increase with the time (p=0.0007). CONCLUSIONS: Colon segments without fecal stream showed high levels of oxidative DNA damage related to histological alterations observed in diversion colitis. The levels of oxidative DNA damage in segments devoid of the fecal stream increase with the time of intestinal exclusion.
Authors: Carlos Augusto Real Martinez; Fabio Guilherme Caserta Maryssael de Campos; Viviel Rodrigo José de Carvalho; Caroline de Castro Ferreira; Murilo Rocha Rodrigues; Daniela Tiemi Sato; José Aires Pereira Journal: J Mol Histol Date: 2015-02-04 Impact factor: 2.611
Authors: Carlos Augusto Real Martinez; Marcos Gonçalves de Almeida; Camila Moraes Gonçalves da Silva; Marcelo Lima Ribeiro; Fernando Lorenzetti da Cunha; Murilo Rocha Rodrigues; Daniela Tiemi Sato; José Aires Pereira Journal: Dig Dis Sci Date: 2013-07-05 Impact factor: 3.199
Authors: Rodrigo Goulart Pacheco; Christiano Costa Esposito; Lucas C M Müller; Morgana T L Castelo-Branco; Leonardo Pereira Quintella; Vera Lucia A Chagas; Heitor Siffert P de Souza; Alberto Schanaider Journal: World J Gastroenterol Date: 2012-08-28 Impact factor: 5.742
Authors: Oscar Orlando Araya Fernandez; José Aires Pereira; Fábio Guilherme Campos; Carolina Mardegan Araya; Gabriele Escocia Marinho; Rafaela de Souza Novo; Thais Silva de Oliveira; Yara Tinoco Franceschi; Carlos Augusto Real Martinez Journal: Arq Bras Cir Dig Date: 2017 Apr-Jun
Authors: Oscar Orlando Araya Fernandez; José Aires Pereira; Fábio Guilherme Campos; Carolina Mardegan Araya; Gabriele Escocia Marinho; Rafaela de Souza Novo; Thais Silva de Oliveira; Yara Tinoco Franceschi; Carlos Augusto Real Martinez Journal: Arq Bras Cir Dig Date: 2018-08-16