Literature DB >> 20877850

Total chemical synthesis of human proinsulin.

Samuel Luisier1, Michal Avital-Shmilovici, Michael A Weiss, Stephen B H Kent.   

Abstract

A convergent synthetic strategy based on modern chemical ligation methods was used to make human proinsulin. The synthetic protein was characterized by LCMS, CD spectroscopy, and by 1D- and 2D-NMR spectroscopy. Synthetic human proinsulin had full biochemical activity in a receptor-binding assay.

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Year:  2010        PMID： 20877850      PMCID： PMC3835593          DOI： 10.1039/c0cc03141k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  13 in total

Review 1.  Synthesis of native proteins by chemical ligation.

Authors:  P E Dawson; S B Kent
Journal:  Annu Rev Biochem       Date:  2000       Impact factor: 23.643

2.  Expression, purification, and PC1-mediated processing of (H10D, P28K, and K29P)-human proinsulin.

Authors:  Robert B Mackin; Meredith H Choquette
Journal:  Protein Expr Purif       Date:  2003-02       Impact factor: 1.650

3.  A one-pot total synthesis of crambin.

Authors:  Duhee Bang; Stephen B H Kent
Journal:  Angew Chem Int Ed Engl       Date:  2004-05-03       Impact factor: 15.336

4.  The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma.

Authors:  D F Steiner; P E Oyer
Journal:  Proc Natl Acad Sci U S A       Date:  1967-02       Impact factor: 11.205

5.  Insulin-proinsulin, a new crystalline complex.

Authors:  B W Low; W W Fullerton; L S Rosen
Journal:  Nature       Date:  1974-03-22       Impact factor: 49.962

6.  Proinsulin: further crystallization and x-ray crystallographic studies of bovine and porcine prohormone.

Authors:  L S Rosen; W W Fullerton; B W Low
Journal:  Arch Biochem Biophys       Date:  1972-10       Impact factor: 4.013

7.  Studies on human proinsulin. Isolation and amino acid sequence of the human pancreatic C-peptide.

Authors:  P E Oyer; S Cho; J D Peterson; D F Steiner
Journal:  J Biol Chem       Date:  1971-03-10       Impact factor: 5.157

8.  Cocrystallization of proinsulin and insulin.

Authors:  D F Steiner
Journal:  Nature       Date:  1973-06-29       Impact factor: 49.962

Review 9.  Molecular effects of proinsulin C-peptide.

Authors:  Jan Johansson; Karin Ekberg; Jawed Shafqat; Mikael Henriksson; Alexander Chibalin; John Wahren; Hans Jörnvall
Journal:  Biochem Biophys Res Commun       Date:  2002-08-02       Impact factor: 3.575

10.  Solution structure of proinsulin: connecting domain flexibility and prohormone processing.

Authors:  Yanwu Yang; Qing-Xin Hua; Jin Liu; Eri H Shimizu; Meredith H Choquette; Robert B Mackin; Michael A Weiss
Journal:  J Biol Chem       Date:  2010-01-27       Impact factor: 5.157
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  3 in total

1.  Fully convergent chemical synthesis of ester insulin: determination of the high resolution X-ray structure by racemic protein crystallography.

Authors:  Michal Avital-Shmilovici; Kalyaneswar Mandal; Zachary P Gates; Nelson B Phillips; Michael A Weiss; Stephen B H Kent
Journal:  J Am Chem Soc       Date:  2013-02-08       Impact factor: 15.419

2.  Deciphering a molecular mechanism of neonatal diabetes mellitus by the chemical synthesis of a protein diastereomer, [D-AlaB8]human proinsulin.

Authors:  Michal Avital-Shmilovici; Jonathan Whittaker; Michael A Weiss; Stephen B H Kent
Journal:  J Biol Chem       Date:  2014-07-07       Impact factor: 5.157

Review 3.  Structural Lessons From the Mutant Proinsulin Syndrome.

Authors:  Balamurugan Dhayalan; Deepak Chatterjee; Yen-Shan Chen; Michael A Weiss
Journal:  Front Endocrinol (Lausanne)       Date:  2021-09-30       Impact factor: 5.555
  3 in total

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