Literature DB >> 20875605

Electrochemical study of gelatin as a matrix for the immobilization of horse heart cytochrome c.

Karolien De Wael1, Stijn De Belder, Sandra Van Vlierberghe, Geert Van Steenberge, Peter Dubruel, Annemie Adriaens.   

Abstract

The aim of this paper is to emphasize the strength of gelatin as a stable matrix for redox enzymes. Cyclic voltammetry has been applied for a detailed electrochemical study of horse heart cytochrome c (HHC) entrapped in a gelatin matrix immobilized on a gold electrode. The influence of the HHC concentration, the mass percentage of the gelatin and the nature of the gelatin on the electrochemical behaviour of HHC have been described in detail. In addition, attenuated total reflection infrared (ATR-IR) spectroscopy was used to prove the immobilization on a qualitative and conformational level. The thickness of the gelatin film was determined using a non-contact optical profiler. These results open up new perspectives in the development of stable, biocompatible matrices for redox enzymes. The latter has its relevance in the field of biosensor development.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20875605     DOI： 10.1016/j.talanta.2010.08.019

Source DB:  PubMed          Journal:  Talanta        ISSN： 0039-9140            Impact factor:   6.057


  7 in total

1.  Antitumor activity of total paeony glycoside against human chronic myelocytic leukemia K562 cell lines in vitro and in vivo.

Authors:  Hui-Yu Xu; Zhi-Wei Chen; Yan-Min Wu
Journal:  Med Oncol       Date:  2011-03-31       Impact factor: 3.064

2.  Pseudoperoxidase activity, conformational stability, and aggregation propensity of the His98Tyr myoglobin variant: implications for the onset of myoglobinopathy.

Authors:  Stefan Hofbauer; Marcello Pignataro; Marco Borsari; Carlo Augusto Bortolotti; Giulia Di Rocco; Gianina Ravenscroft; Paul G Furtmüller; Christian Obinger; Marco Sola; Gianantonio Battistuzzi
Journal:  FEBS J       Date:  2021-11-03       Impact factor: 5.622

3.  An improved electrochemical aptasensor for chloramphenicol detection based on aptamer incorporated gelatine.

Authors:  Ezat Hamidi-Asl; Freddy Dardenne; Ronny Blust; Karolien De Wael
Journal:  Sensors (Basel)       Date:  2015-03-27       Impact factor: 3.576

4.  Enzyme-gelatin electrochemical biosensors: scaling down.

Authors:  Karolien De Wael; Stijn De Belder; Sanaz Pilehvar; Geert Van Steenberge; Wouter Herrebout; Hendrik A Heering
Journal:  Biosensors (Basel)       Date:  2012-03-15

5.  A redox signalling globin is essential for reproduction in Caenorhabditis elegans.

Authors:  Sasha De Henau; Lesley Tilleman; Matthew Vangheel; Evi Luyckx; Stanislav Trashin; Martje Pauwels; Francesca Germani; Caroline Vlaeminck; Jacques R Vanfleteren; Wim Bert; Alessandra Pesce; Marco Nardini; Martino Bolognesi; Karolien De Wael; Luc Moens; Sylvia Dewilde; Bart P Braeckman
Journal:  Nat Commun       Date:  2015-12-01       Impact factor: 14.919

6.  Bio-Nanohybrid Gelatin/Quantum Dots for Cellular Imaging and Biosensing Applications.

Authors:  Sangram Keshari Samal; Stefaan Soenen; Dario Puppi; Karolien De Wael; Sanghamitra Pati; Stefaan De Smedt; Kevin Braeckmans; Peter Dubruel
Journal:  Int J Mol Sci       Date:  2022-10-06       Impact factor: 6.208

7.  An N-myristoylated globin with a redox-sensing function that regulates the defecation cycle in Caenorhabditis elegans.

Authors:  Lesley Tilleman; Sasha De Henau; Martje Pauwels; Nora Nagy; Isabel Pintelon; Bart P Braeckman; Karolien De Wael; Sabine Van Doorslaer; Dirk Adriaensen; Jean-Pierre Timmermans; Luc Moens; Sylvia Dewilde
Journal:  PLoS One       Date:  2012-12-12       Impact factor: 3.240
  7 in total

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