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Literature DB >> 20875397

A functional interaction between CPI-17 and RACK1 proteins in bronchial smooth muscle cells.

Yoshihiko Chiba¹, Miki Tanabe, Hiroyasu Sakai, Shioko Kimura, Miwa Misawa.

Abstract

CPI-17 is a phosphorylation-dependent inhibitor of smooth muscle myosin light chain. Using yeast two-hybrid system, we have identified the receptor for activated C kinase 1 (RACK1) as a novel interaction partner of CPI-17. The direct interaction and co-localization of CPI-17 with RACK1 were confirmed by immunoprecipitation and confocal microscopy analysis, respectively. An in vitro assay system using recombinant/purified proteins revealed that the PKC-mediated phosphorylation of CPI-17 was augmented in the presence of RACK1. These results suggest that RACK1 may play a role in PKC/CPI-17 signaling pathway.

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Year: 2010 PMID： 20875397 PMCID： PMC3486735 DOI： 10.1016/j.bbrc.2010.09.086

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

18 in total

1. Expression of CPI-17 and myosin phosphatase correlates with Ca(2+) sensitivity of protein kinase C-induced contraction in rabbit smooth muscle.

Authors: T P Woodsome; M Eto; A Everett; D L Brautigan; T Kitazawa
Journal: J Physiol Date: 2001-09-01 Impact factor: 5.182

2. Cerebellar long-term synaptic depression requires PKC-mediated activation of CPI-17, a myosin/moesin phosphatase inhibitor.

Authors: Masumi Eto; Roland Bock; David L Brautigan; David J Linden
Journal: Neuron Date: 2002-12-19 Impact factor: 17.173

3. A novel protein phosphatase-1 inhibitory protein potentiated by protein kinase C. Isolation from porcine aorta media and characterization.

Authors: M Eto; T Ohmori; M Suzuki; K Furuya; F Morita
Journal: J Biochem Date: 1995-12 Impact factor: 3.387

Review 4. The role of RhoA-mediated Ca2+ sensitization of bronchial smooth muscle contraction in airway hyperresponsiveness.

Authors: Yoshihiko Chiba; Miwa Misawa
Journal: J Smooth Muscle Res Date: 2004-10

5. Molecular cloning of a novel phosphorylation-dependent inhibitory protein of protein phosphatase-1 (CPI17) in smooth muscle: its specific localization in smooth muscle.

Authors: M Eto; S Senba; F Morita; M Yazawa
Journal: FEBS Lett Date: 1997-06-30 Impact factor: 4.124

6. Possible involvement of the novel CPI-17 protein in protein kinase C signal transduction of rabbit arterial smooth muscle.

Authors: L Li; M Eto; M R Lee; F Morita; M Yazawa; T Kitazawa
Journal: J Physiol Date: 1998-05-01 Impact factor: 5.182

7. RACK1 promotes breast carcinoma migration/metastasis via activation of the RhoA/Rho kinase pathway.

Authors: Xi-Xi Cao; Jing-Da Xu; Jia-Wen Xu; Xiao-Li Liu; Yuan-Yuan Cheng; Qing-Quan Li; Zu-De Xu; Xiu-Ping Liu
Journal: Breast Cancer Res Treat Date: 2010-05-25 Impact factor: 4.872

8. Reconstitution of protein kinase C-induced contractile Ca2+ sensitization in triton X-100-demembranated rabbit arterial smooth muscle.

Authors: T Kitazawa; N Takizawa; M Ikebe; M Eto
Journal: J Physiol Date: 1999-10-01 Impact factor: 5.182