| Literature DB >> 20873767 |
Fan Xia1, Ryan J White, Xiaolei Zuo, Adriana Patterson, Yi Xiao, Di Kang, Xiong Gong, Kevin W Plaxco, Alan J Heeger.
Abstract
In a traditional sandwich assay, a DNA target hybridizes to a single copy of the signal probe. Here we employ a modified signal probe containing a methylene blue (a redox moiety) label and a "sticky end." When a DNA target hybridizes this signal probe, the sticky end remains free to hybridize another target leading to the creation of a supersandwich structure containing multiple labels. This leads to large signal amplification upon monitoring by voltammetry.Entities:
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Year: 2010 PMID： 20873767 PMCID： PMC3082473 DOI： 10.1021/ja104998m
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419