Qiu-shi Chen1, Jie Wu, Wei Li, Bei Cheng. 1. Department of Pharmaceutical Engineering, Wuhan Bioengineering Institute, Wuhan 430415, China. chenqiushi@yahoo.cn
Abstract
OBJECTIVE: To select the best extraction of oleanolic acid from leaves of Chaenomeles speciosa and processing techniques of its HP-beta-cyclodextrin (HP-beta-CD) inclusion compound. METHODS: The study was carried out with orthogonal experiment. Ultrasonic treatment time, extraction time, temperature of extraction and ratio of material to liquor were used as indices. The optimum inclusion technique was chosen by determining the oleanolic acid-bearing rate. The processing techniques of the HP-beta-CD inclusion compound was optimized by taking the ratio of HP-beta-CD to oleanolic acid, the time and temperature of the inclusion process as factors and with the yield of the inclusion compound and the inclusion rate of the volatile oil as the indices. RESULTS: The optimal process for oleanolic acid extraction were as following: ultrasonic treatment time as 40 min, extraction time as 70 min, temperature of extraction as 60 degrees C, ratio of material to liquor as 1:10. The optimal processing conditions for HP-beta-CD inclusion compound of oleanolic acid were as following: the ratio of HP-beta-CD to oleanolic acid was 1:2, the inclusion time of the compound was 3.0 hours at 60 degrees C. CONCLUSION: High extraction yield of oleanolic acid and stable inclusion compound can be obtained using the above method.
OBJECTIVE: To select the best extraction of oleanolic acid from leaves of Chaenomeles speciosa and processing techniques of its HP-beta-cyclodextrin (HP-beta-CD) inclusion compound. METHODS: The study was carried out with orthogonal experiment. Ultrasonic treatment time, extraction time, temperature of extraction and ratio of material to liquor were used as indices. The optimum inclusion technique was chosen by determining the oleanolic acid-bearing rate. The processing techniques of the HP-beta-CD inclusion compound was optimized by taking the ratio of HP-beta-CD to oleanolic acid, the time and temperature of the inclusion process as factors and with the yield of the inclusion compound and the inclusion rate of the volatile oil as the indices. RESULTS: The optimal process for oleanolic acid extraction were as following: ultrasonic treatment time as 40 min, extraction time as 70 min, temperature of extraction as 60 degrees C, ratio of material to liquor as 1:10. The optimal processing conditions for HP-beta-CD inclusion compound of oleanolic acid were as following: the ratio of HP-beta-CD to oleanolic acid was 1:2, the inclusion time of the compound was 3.0 hours at 60 degrees C. CONCLUSION: High extraction yield of oleanolic acid and stable inclusion compound can be obtained using the above method.