BACKGROUND: Reports have identified cells capable of osteogenic differentiation in bone marrow, muscle, and adipose tissues, but there are few direct comparisons of these different cell types. Also, few have investigated the potential connection between a tissue-specific abnormality and cells derived from seemingly unrelated tissues. In this article, the authors compare cells isolated from wild-type rabbits or rabbits with nonsyndromic craniosynostosis, defined as the premature fusion of one or more of the cranial sutures. METHODS: Cells were derived from bone marrow, adipose, and muscle of 10-day-old wild-type rabbits (n = 17) or from age-matched rabbits with familial nonsyndromic craniosynostosis (n = 18). Cells were stimulated with bone morphogenetic protein-4 (BMP4), and alkaline phosphatase expression and cell proliferation were assessed. RESULTS: In wild-type rabbits, cells derived from muscle had more alkaline phosphatase activity than cells derived from either adipose or bone marrow. The cells derived from craniosynostotic rabbit bone marrow and muscle were significantly more osteogenic than those derived from wild-type rabbits. Adipose-derived cells demonstrated no significant differences. Although muscle-derived cells were most osteogenic in wild-type rabbits, bone marrow-derived cells were most osteogenic in craniosynostotic rabbits. CONCLUSIONS: These results suggest that cells from different tissues have different potentials for differentiation. Furthermore, cells derived from rabbits with craniosynostosis were different from cells from wild-type rabbits. Interestingly, cells derived from the craniosynostotic rabbits were not uniformly more responsive compared with wild-type cells, suggesting that specific tissue-derived cells may react differently in individuals with craniosynostosis.
BACKGROUND: Reports have identified cells capable of osteogenic differentiation in bone marrow, muscle, and adipose tissues, but there are few direct comparisons of these different cell types. Also, few have investigated the potential connection between a tissue-specific abnormality and cells derived from seemingly unrelated tissues. In this article, the authors compare cells isolated from wild-type rabbits or rabbits with nonsyndromic craniosynostosis, defined as the premature fusion of one or more of the cranial sutures. METHODS: Cells were derived from bone marrow, adipose, and muscle of 10-day-old wild-type rabbits (n = 17) or from age-matched rabbits with familial nonsyndromic craniosynostosis (n = 18). Cells were stimulated with bone morphogenetic protein-4 (BMP4), and alkaline phosphatase expression and cell proliferation were assessed. RESULTS: In wild-type rabbits, cells derived from muscle had more alkaline phosphatase activity than cells derived from either adipose or bone marrow. The cells derived from craniosynostotic rabbit bone marrow and muscle were significantly more osteogenic than those derived from wild-type rabbits. Adipose-derived cells demonstrated no significant differences. Although muscle-derived cells were most osteogenic in wild-type rabbits, bone marrow-derived cells were most osteogenic in craniosynostotic rabbits. CONCLUSIONS: These results suggest that cells from different tissues have different potentials for differentiation. Furthermore, cells derived from rabbits with craniosynostosis were different from cells from wild-type rabbits. Interestingly, cells derived from the craniosynostotic rabbits were not uniformly more responsive compared with wild-type cells, suggesting that specific tissue-derived cells may react differently in individuals with craniosynostosis.
Authors: Richard K Burt; Yvonne Loh; Bruce Cohen; Dusan Stefoski; Dusan Stefosky; Roumen Balabanov; George Katsamakis; Yu Oyama; Eric J Russell; Jessica Stern; Paolo Muraro; John Rose; Alessandro Testori; Jurate Bucha; Borko Jovanovic; Francesca Milanetti; Jan Storek; Julio C Voltarelli; William H Burns Journal: Lancet Neurol Date: 2009-01-29 Impact factor: 44.182
Authors: Lieven Herbots; Jan D'hooge; Elif Eroglu; Daisy Thijs; Javier Ganame; Piet Claus; Christophe Dubois; Koen Theunissen; Jan Bogaert; Joseph Dens; Maria Kalantzi; Steven Dymarkowski; Bart Bijnens; Ann Belmans; Marc Boogaerts; George Sutherland; Frans Van de Werf; Frank Rademakers; Stefan Janssens Journal: Eur Heart J Date: 2008-12-23 Impact factor: 29.983
Authors: H Strasser; R Marksteiner; E Margreiter; M Mitterberger; G M Pinggera; F Frauscher; M Fussenegger; K Kofler; G Bartsch Journal: World J Urol Date: 2007-08-14 Impact factor: 4.226
Authors: Júlio C Voltarelli; Carlos E B Couri; Ana B P L Stracieri; Maria C Oliveira; Daniela A Moraes; Fabiano Pieroni; Marina Coutinho; Kelen C R Malmegrim; Maria C Foss-Freitas; Belinda P Simões; Milton C Foss; Elizabeth Squiers; Richard K Burt Journal: JAMA Date: 2007-04-11 Impact factor: 56.272