Literature DB >> 20870470

Qualitative and quantitative determination of major saponins in Paris and Trillium by HPLC-ELSD and HPLC-MS/MS.

Shuli Man1, Wenyuan Gao, Yanjun Zhang, Jieyin Wang, Wanshun Zhao, Luqi Huang, Changxiao Liu.   

Abstract

High-performance liquid chromatographic (HPLC) with evaporative light scattering detection (ELSD) and HPLC with electrospray ionization multistage tandem mass spectrometry (HPLC-ESI-MS(n)) were used to identify and quantify steroid saponins in Paris and Trillium plants. The content of the known saponins such as Paris I, II, III, V, VI, VII, H, gracillin and protodioscin in Paris and Trillium plants was determined simultaneously using the developed HPLC-ELSD method. Furthermore, other 12 steroid saponins were identified by HPLC-ESI(+/-)-MS(n) detection. In the end, a developed analytical procedure was proved to be a reliable and rapid method for the quality control of Paris and Trillium plants. In addition, the alternative resources for Paris yunnanensis used as a traditional Chinese medicine were discovered according to the hierarchical clustering analysis of the saponin fraction of these plants.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20870470     DOI： 10.1016/j.jchromb.2010.08.033

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  5 in total

1.  Systematic Qualitative and Quantitative Analyses of Wenxin Granule via Ultra-High Performance Liquid Chromatography Coupled with Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry and Triple Quadrupole-Linear Ion Trap Mass Spectrometry.

Authors:  Yueguang Mi; Wandi Hu; Weiwei Li; Shiyu Wan; Xiaoyan Xu; Meiyu Liu; Hongda Wang; Quanxi Mei; Qinhua Chen; Yang Yang; Boxue Chen; Meiting Jiang; Xue Li; Wenzhi Yang; Dean Guo
Journal:  Molecules       Date:  2022-06-06       Impact factor: 4.927

2.  Preparation of progenin III from total steroidal saponins of Dioscorea nipponica Makino using a crude enzyme from Aspergillus oryzae strain.

Authors:  Tingqiang Liu; Hongshan Yu; Chunying Liu; Yongming Bao; Xiangchun Hu; Yuanhao Wang; Bing Liu; Yaoyao Fu; Sihui Tang; Fengxie Jin
Journal:  J Ind Microbiol Biotechnol       Date:  2013-03-08       Impact factor: 3.346

3.  Pennogenyl Saponins from Paris quadrifolia L. Induce Extrinsic and Intrinsic Pathway of Apoptosis in Human Cervical Cancer HeLa Cells.

Authors:  Justyna Stefanowicz-Hajduk; Rafal Bartoszewski; Sylwia Bartoszewska; Kinga Kochan; Anna Adamska; Igor Kosiński; J Renata Ochocka
Journal:  PLoS One       Date:  2015-08-21       Impact factor: 3.240

4.  RNA-Seq analysis reveals the important co-expressed genes associated with polyphyllin biosynthesis during the developmental stages of Paris polyphylla.

Authors:  Xiaoyang Gao; Qixuan Su; Jing Li; Wenjing Yang; Baolin Yao; Jiawei Guo; Shengying Li; Changning Liu
Journal:  BMC Genomics       Date:  2022-08-05       Impact factor: 4.547

5.  Isolation and identification of cytotoxic compounds from the rhizomes of Paris quadrifolia L.

Authors:  Jerzy Gajdus; Zbigniew Kaczyński; Anna Kawiak; Ewa Lojkowska; Justyna Stefanowicz-Hajduk; J Renata Ochocka; Piotr Stepnowski
Journal:  Pharmacogn Mag       Date:  2014-04       Impact factor: 1.085
  5 in total

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