Tian Ding1, Xinyu Wang, Feng Ye, Xiaodong Cheng, Ding Ma, Weiguo Lu, Xing Xie. 1. Women's Reproductive Health Laboratory of Zhejiang Province and Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, People's Republic of China.
Abstract
OBJECTIVES: The specific intratype HPV genome variations may be associated with the development of cervical cancer in specific geographic regions and a given population. Human papillomavirus (HPV) 58 and 52 have been found to be relatively prevalent among Asian women including Chinese women. This study aimed to assess the risk of HPV 58 and 52 variants for cervical cancer and its precursors in Chinese women. METHODS: A total of 2021cervical samples were collected. After DNA extraction and genotyping, a total of 298 (177 HPV58-single positive and 121 HPV52-single positive) DNA samples were analyzed for E6 and E7 sequence variations by direct sequencing. RESULTS: A total of 29 new reported variations of HPV 58 and 52 were found. For HPV58, the presence of C632T (T20I) and G760A (G63S) variants in E7 showed a positive trend of the association with the severity of neoplasia (P(trend)<0.05, χ² test for trend). CONCLUSIONS: These findings suggest that C632T (T20I) and G760A (G63S) variants in HPV58 E7 are probably risk factors associated with the development of cervical cancer in Chinese women. The presence of HPV58/52 E6 and E7 variants may be different in Chinese women.
OBJECTIVES: The specific intratype HPV genome variations may be associated with the development of cervical cancer in specific geographic regions and a given population. Human papillomavirus (HPV) 58 and 52 have been found to be relatively prevalent among Asian women including Chinese women. This study aimed to assess the risk of HPV 58 and 52 variants for cervical cancer and its precursors in Chinese women. METHODS: A total of 2021cervical samples were collected. After DNA extraction and genotyping, a total of 298 (177 HPV58-single positive and 121 HPV52-single positive) DNA samples were analyzed for E6 and E7 sequence variations by direct sequencing. RESULTS: A total of 29 new reported variations of HPV 58 and 52 were found. For HPV58, the presence of C632T (T20I) and G760A (G63S) variants in E7 showed a positive trend of the association with the severity of neoplasia (P(trend)<0.05, χ² test for trend). CONCLUSIONS: These findings suggest that C632T (T20I) and G760A (G63S) variants in HPV58 E7 are probably risk factors associated with the development of cervical cancer in Chinese women. The presence of HPV58/52 E6 and E7 variants may be different in Chinese women.
Authors: Chuqing Zhang; Jong-Sup Park; Magdalena Grce; Samantha Hibbitts; Joel M Palefsky; Ryo Konno; Karen K Smith-McCune; Lucia Giovannelli; Tang-Yuan Chu; María Alejandra Picconi; Patricia Piña-Sánchez; Wannapa Settheetham-Ishida; Francois Coutlée; Federico De Marco; Yin-Ling Woo; Wendy C S Ho; Martin C S Wong; Mike Z Chirenje; Tsitsi Magure; Anna-Barbara Moscicki; Ivan Sabol; Alison N Fiander; Zigui Chen; Martin C W Chan; Tak-Hong Cheung; Robert D Burk; Paul K S Chan Journal: J Infect Dis Date: 2014-05-30 Impact factor: 5.226
Authors: Paul K S Chan; Chuqing Zhang; Jong-Sup Park; Karen K Smith-McCune; Joel M Palefsky; Lucia Giovannelli; Francois Coutlée; Samantha Hibbitts; Ryo Konno; Wannapa Settheetham-Ishida; Tang-Yuan Chu; Annabelle Ferrera; María Alejandra Picconi; Federico De Marco; Yin-Ling Woo; Tainá Raiol; Patricia Piña-Sánchez; Jeong-Hoon Bae; Martin C S Wong; Mike Z Chirenje; Tsitsi Magure; Anna-Barbara Moscicki; Alison N Fiander; Giuseppina Capra; Eun Young Ki; Yi Tan; Zigui Chen; Robert D Burk; Martin C W Chan; Tak-Hong Cheung; David Pim; Lawrence Banks Journal: Int J Cancer Date: 2012-11-29 Impact factor: 7.396
Authors: Zigui Chen; Wendy C S Ho; Siaw Shi Boon; Priscilla T Y Law; Martin C W Chan; Rob DeSalle; Robert D Burk; Paul K S Chan Journal: J Virol Date: 2017-10-13 Impact factor: 5.103