Transmission electron microscopy of cartilage and bone.

This review of cartilage microscopy is restricted to a brief description of the major molecular components in cartilage and bone and an in-depth discussion of methods for the preparation of these tissues for examination by transmission electron microscopy. Included within are simple methods for the isolation of cartilage-specific macromolecules, methods for immunoidentification of tissue components, bone decalcification protocols, and fixative recipes designed to stabilize cells and proteoglycan within the cartilage matrix. A discussion of cartilage prepared by high-pressure freezing (HPF)/freeze substitution (FS) is also presented.