Literature DB >> 20868327

Shear stress induces osteogenic differentiation of human mesenchymal stem cells.

Gregory Yourek1, Susan M McCormick, Jeremy J Mao, Gwendolen C Reilly.   

Abstract

AIM: To determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells. MATERIALS &
METHODS: hMSCs cultured with or without osteogenic differentiation medium were exposed to fluid flow-induced shear stress and analyzed for alkaline phosphatase activity and expression of osteogenic genes.
RESULTS: Immediately following shear stress, alkaline phosphatase activity in osteogenic medium was significantly increased. At days 4 and 8 of culture the mRNA expression of bone morphogenetic protein-2 and osteopontin was significantly higher in hMSCs subjected to shear stress than those cultured in static conditions. However, hMSCs cultured in osteogenic differentiation medium were less responsive in gene expression of alkaline phosphatase and bone morphogenetic protein-2.
CONCLUSION: These data demonstrate that shear stress stimulates hMSCs towards an osteoblastic phenotype in the absence of chemical induction, suggesting that certain mechanical stresses may serve as an alternative to chemical stimulation of stem cell differentiation.

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Year:  2010        PMID： 20868327      PMCID： PMC4093787          DOI： 10.2217/rme.10.60

Source DB:  PubMed          Journal:  Regen Med        ISSN： 1746-0751            Impact factor:   3.806


  57 in total

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