Crossover behavior in static and dynamic properties of a single DNA molecule from three to quasi-two dimensions.

We studied the conformation and dynamics of a single DNA molecule in a thin slit by a fluorescent microscope. In a slit thinner than the Flory radius in three dimensions, the length of the major axis, the translational self-diffusion coefficient and the rotational relaxation time in a dilute solution show the apparent dependence on the thickness of the slit. The observed dependence is in agreement with that predicted by blob theory, despite the number of blobs is very small. The radial distribution of the segments around the center of mass of a single molecule was also studied and compared with that calculated for a Gaussian and an excluded volume chain. The influence of the polymer concentration on the geometrical confinement by slits was also studied in a semidilute solution near the overlap concentration c∗. The confinement effect is found to be not so serious near c∗ and is only significant in the so-called "two-dimensional pancake" region.