| Literature DB >> 20865363 |
Dorota Butkiewicz1, Małgorzata Krześniak, Rasa Vaitiekunaite, Bozena Sikora, Elise D Bowman, Curtis C Harris, Marek Rusin.
Abstract
The XPA gene has a commonly occurring polymorphism (G23A) associated with cancer risk. This study assessed the functional significance of this polymorphism, which is localised near the translation start codon. Lymphoblastoid cell lines with alternative homozygous genotypes showed no significant differences in their XPA levels. The luciferase reporter assay detected no functional difference between the two sequences. Unexpectedly, we found that the alternatively spliced form of XPA mRNA lacked a part of exon 1. Only the reading frame downstream of codon Met59 was preserved. The alternative mRNA is expressed in various human tissues. The analysis of the 5'cDNA ends showed similar transcription start sites for the two forms. The in vitro expression of the alternative XPA labelled with the red fluorescent protein (mRFP) showed a lack of preferential nuclear accumulation of the XPA isoform. The biological role of the alternative XPA mRNA form remains to be elucidated.Entities:
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Year: 2010 PMID： 20865363 PMCID： PMC6275895 DOI： 10.2478/s11658-010-0032-2
Source DB: PubMed Journal: Cell Mol Biol Lett ISSN： 1425-8153 Impact factor: 5.787