OBJECTIVE: To compare the ultrastructural appearance of oocytes after vitrification and warming with two different devices. DESIGN: Oocytes were examined by ultrastructural analysis after vitrification and warming with use of closed (CryoTip; Irvine Scientific, Santa Ana, CA) or open (Cryotop; Kitazato BioPharma Co., Ltd., Shizuoka, Japan) devices. SETTING: Pordenone Hospital IVF Unit and Medical Morphological Research Department, University of Udine. PATIENT(S): Surplus oocytes from 10 patients (aged 31-39 years) undergoing assisted reproductive technologies at the Pathophysiology Unit of Human Reproduction and Sperm Bank between 2006 and 2008. INTERVENTION(S): Oocytes with normal invertoscopic appearance underwent vitrification and warming with closed (CryoTip) or open (Cryotop) devices and were processed for transmission electron microscopy. MAIN OUTCOME MEASURE(S): Cryodamage extent and cell alterations in oocytes after open or closed vitrification and warming procedures and their rehydration rate. RESULT(S): A higher rate of complete oocyte rehydration and less-severe ultrastructural alterations were observed after vitrification and warming with the open Cryotop device. CONCLUSION(S): These preliminary data suggest that oocyte ultrastructure is better preserved with an open rather than closed vitrification and warming protocol.
OBJECTIVE: To compare the ultrastructural appearance of oocytes after vitrification and warming with two different devices. DESIGN: Oocytes were examined by ultrastructural analysis after vitrification and warming with use of closed (CryoTip; Irvine Scientific, Santa Ana, CA) or open (Cryotop; Kitazato BioPharma Co., Ltd., Shizuoka, Japan) devices. SETTING: Pordenone Hospital IVF Unit and Medical Morphological Research Department, University of Udine. PATIENT(S): Surplus oocytes from 10 patients (aged 31-39 years) undergoing assisted reproductive technologies at the Pathophysiology Unit of Human Reproduction and Sperm Bank between 2006 and 2008. INTERVENTION(S): Oocytes with normal invertoscopic appearance underwent vitrification and warming with closed (CryoTip) or open (Cryotop) devices and were processed for transmission electron microscopy. MAIN OUTCOME MEASURE(S): Cryodamage extent and cell alterations in oocytes after open or closed vitrification and warming procedures and their rehydration rate. RESULT(S): A higher rate of complete oocyte rehydration and less-severe ultrastructural alterations were observed after vitrification and warming with the open Cryotop device. CONCLUSION(S): These preliminary data suggest that oocyte ultrastructure is better preserved with an open rather than closed vitrification and warming protocol.
Authors: Christian S Ottolini; Antonio Capalbo; Louise Newnham; Danilo Cimadomo; Senthilkumar A Natesan; Eva R Hoffmann; Filippo M Ubaldi; Laura Rienzi; Alan H Handyside Journal: Nat Protoc Date: 2016-06-16 Impact factor: 13.491
Authors: Cassie T Wang; Lifeng Liang; Craig Witz; Dan Williams; Jason Griffith; Josh Skorupski; Ghassan Haddad; Jimmy Gill; Weihua Wang Journal: J Ovarian Res Date: 2013-02-13 Impact factor: 4.234