Y Yang1, D Dong, L Peng, J Ling, Y Xiao, H Zhuang. 1. Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, P.R.China.
Abstract
BACKGROUND: To research the relationship between human papillomavirus (HPV) infection and the oncogenesis of primary squamous carcinoma of the lung. METHODS: 50 formalin-fixed paraffin-embedded tissues of squamous cell carcinoma of the lung , 30 samples of areas of squamous epithelium metaplasia near tumors , and 30 samples of normal bronchial mucosa were detected for the presence of HPV DNA by polymerase chain reaction (PCR) . RESULTS: In these samples , the positive detection rate of HPV DNA was 26%, 36.7% and 10% respectively. Of the detected HPV DNA , HPV16 was of the highest ratio. The positive rate of HPV6/11 and HPV16 in squamous cell carcinoma and squamous epithelium metaplasia had no significant difference by Chi-Square test ( P > 0. 05) . CONCLUSIONS: These results suggest that HPV infection might play an important role in the oncogenesis of squamous cell carcinomas of the lung , and squamous epithelium metaplasia of the bronchus could be regarded as a precancer lesion. Compared with biotinylated DNA probes and hybridization technique , PCR has a higher sensitivity.
BACKGROUND: To research the relationship between human papillomavirus (HPV) infection and the oncogenesis of primary squamous carcinoma of the lung. METHODS: 50 formalin-fixed paraffin-embedded tissues of squamous cell carcinoma of the lung , 30 samples of areas of squamous epithelium metaplasia near tumors , and 30 samples of normal bronchial mucosa were detected for the presence of HPV DNA by polymerase chain reaction (PCR) . RESULTS: In these samples , the positive detection rate of HPV DNA was 26%, 36.7% and 10% respectively. Of the detected HPV DNA , HPV16 was of the highest ratio. The positive rate of HPV6/11 and HPV16 in squamous cell carcinoma and squamous epithelium metaplasia had no significant difference by Chi-Square test ( P > 0. 05) . CONCLUSIONS: These results suggest that HPV infection might play an important role in the oncogenesis of squamous cell carcinomas of the lung , and squamous epithelium metaplasia of the bronchus could be regarded as a precancer lesion. Compared with biotinylated DNA probes and hybridization technique , PCR has a higher sensitivity.
Authors: Talita Helena Araujo de Oliveira; Carolina Medeiros do Amaral; Bianca de França São Marcos; Kamylla Conceição Gomes Nascimento; Ana Carine de Miranda Rios; Dafne Carolina Alves Quixabeira; Maria Tereza Cartaxo Muniz; Jacinto da Costa Silva Neto; Antonio Carlos de Freitas Journal: J Cancer Res Clin Oncol Date: 2018-09-17 Impact factor: 4.553