Literature DB >> 20862050

Three-dimensional analysis by a microlens-array confocal arrangement.

H J Tiziani, H M Uhde.   

Abstract

Scanning confocal microscopy is now well developed and applied. As an alternative to a laser spot to be scanned, parallel processing can be obtained when a two-dimensional structure is moved through the focal plane and a series of image sections is recorded. Surface topography is determined by analysis of the normalized intensity of the appropriate image points, i.e., a search of the intensity maximum leads to surface coordinates. With a high numerical aperture of the optical system, the half-width of I(z) is small, and the topography can be calculated with high accuracy. But with a high numerical aperture, only small object fields can be reproduced. As an alternative to the Nipkow disk for parallel processing, high-numerical-aperture microlenses are combined in an array. The reproducible object field is then limited by the size of the array and the number of lens and detector elements.

Year:  1994        PMID: 20862050     DOI: 10.1364/AO.33.000567

Source DB:  PubMed          Journal:  Appl Opt        ISSN: 1559-128X            Impact factor:   1.980


  2 in total

Review 1.  Optical microscopy in photosynthesis.

Authors:  Richard Cisek; Leigh Spencer; Nicole Prent; Donatas Zigmantas; George S Espie; Virginijus Barzda
Journal:  Photosynth Res       Date:  2009-10-23       Impact factor: 3.573

2.  High-throughput multispot single-molecule spectroscopy.

Authors:  Ryan A Colyer; Giuseppe Scalia; Taiho Kim; Ivan Rech; Daniele Resnati; Stefano Marangoni; Massimo Ghioni; Sergio Cova; Shimon Weiss; Xavier Michalet
Journal:  Proc SPIE Int Soc Opt Eng       Date:  2010-01-23
  2 in total

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