BACKGROUND: Metastases formation depends on the ability of tumor cells to invade basement membranes in a process involving enzymes capable of degrading extracellular matrix components. METHODS: We examined the expression of heparanase in oral carcinomas and correlated its staining extent, intensity, and cellular localization with patients' outcome. RESULTS: Quantitative real-time polymerase chain reaction (PCR) revealed over 4-fold increase in heparanase levels in oral carcinomas compared to adjacent normal tissue. Normal oral epithelium was found negative for heparanase, while all oral carcinomas stained positively for heparanase. Heparanase staining was associated with Ki67 staining, a measure of cell proliferation. Notably, whereas cytoplasmic localization of heparanase was associated with high-grade carcinomas, nuclear localization of the enzyme was found primarily in low-grade, well-differentiated tumors, and in all oral verrucous carcinomas. CONCLUSION: Expression level and cellular localization of heparanase could serve as an important diagnostic marker in patients with oral cancer.
BACKGROUND:Metastases formation depends on the ability of tumor cells to invade basement membranes in a process involving enzymes capable of degrading extracellular matrix components. METHODS: We examined the expression of heparanase in oral carcinomas and correlated its staining extent, intensity, and cellular localization with patients' outcome. RESULTS: Quantitative real-time polymerase chain reaction (PCR) revealed over 4-fold increase in heparanase levels in oral carcinomas compared to adjacent normal tissue. Normal oral epithelium was found negative for heparanase, while all oral carcinomas stained positively for heparanase. Heparanase staining was associated with Ki67 staining, a measure of cell proliferation. Notably, whereas cytoplasmic localization of heparanase was associated with high-grade carcinomas, nuclear localization of the enzyme was found primarily in low-grade, well-differentiated tumors, and in all oral verrucous carcinomas. CONCLUSION: Expression level and cellular localization of heparanase could serve as an important diagnostic marker in patients with oral cancer.
Authors: Y Nadir; B Brenner; A Zetser; N Ilan; I Shafat; E Zcharia; O Goldshmidt; I Vlodavsky Journal: J Thromb Haemost Date: 2006-09-13 Impact factor: 5.824
Authors: Anna Zetser; Yulia Bashenko; Evgeny Edovitsky; Flonia Levy-Adam; Israel Vlodavsky; Neta Ilan Journal: Cancer Res Date: 2006-02-01 Impact factor: 12.701
Authors: Uri Barash; Victoria Cohen-Kaplan; Ilana Dowek; Ralph D Sanderson; Neta Ilan; Israel Vlodavsky Journal: FEBS J Date: 2010-08-31 Impact factor: 5.542
Authors: Adi Binder Gallimidi; Stuart Fischman; Brurya Revach; Raanan Bulvik; Alina Maliutina; Ariel M Rubinstein; Gabriel Nussbaum; Michael Elkin Journal: Oncotarget Date: 2015-09-08