Literature DB >> 2085141

Calcium mobilization in activated mast cells monitored by flow cytometric analysis.

G L Rossi1, D J Young, S I Wasserman, K E Barrett.   

Abstract

Changes in intracellular calcium levels induced in mast cells by either antigen or ionomycin were monitored using flow cytometry and the calcium binding dye indo-1. Both stimuli increased calcium levels in responsive cells by a similar amount, but not all cells examined were responsive to antigen. Antigen-induced increases in intracellular calcium levels could be completely blocked by the calcium antagonist TMB-8. Flow cytometry appears to be a useful method for monitoring mast cell calcium concentrations, as it provides the capability to study large numbers of individual cells.

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Year:  1990        PMID： 2085141     DOI： 10.1007/BF01997617

Source DB:  PubMed          Journal:  Agents Actions        ISSN： 0065-4299


  13 in total

1.  Measurement of cytoplasmic calcium in lymphocytes using flow cytometry. Kinetic studies and single cell analysis.

Authors:  A W Griffioen; G T Rijkers; J Keij; B J Zegers
Journal:  J Immunol Methods       Date:  1989-06-02       Impact factor: 2.303

2.  Inhibition of mast cell adenosine responsiveness by chronic exposure to adenosine receptor agonists.

Authors:  D L Marquardt; L L Walker
Journal:  Biochem Pharmacol       Date:  1987-12-15       Impact factor: 5.858

3.  Effect of TMB-8 on histamine release from isolated rat mast cells.

Authors:  N Grossman
Journal:  Int Arch Allergy Appl Immunol       Date:  1986

4.  Heterogeneity among T cells in intracellular free calcium responses after mitogen stimulation with PHA or anti-CD3. Simultaneous use of indo-1 and immunofluorescence with flow cytometry.

Authors:  P S Rabinovitch; C H June; A Grossmann; J A Ledbetter
Journal:  J Immunol       Date:  1986-08-01       Impact factor: 5.422

5.  Effect of TMB-8 on calcium transport in mast cells in relation to histamine secretion.

Authors:  N Chakravarty
Journal:  Agents Actions       Date:  1988-04

6.  Imaging asynchronous changes in intracellular Ca2+ in individual stimulated tumor mast cells.

Authors:  P J Millard; D Gross; W W Webb; C Fewtrell
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

7.  Flow cytometric measurements of cytoplasmic calcium changes in human platelets.

Authors:  T A Davies; D Drotts; G J Weil; E R Simons
Journal:  Cytometry       Date:  1988-03

8.  Inhibition of allergic and non-allergic histamine secretion from rat peritoneal mast cells by calcium antagonists.

Authors:  N Chand; W Diamantis; J Pillar; R D Sofia
Journal:  Br J Pharmacol       Date:  1984-12       Impact factor: 8.739

9.  Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.

Authors:  R Y Tsien; T Pozzan; T J Rink
Journal:  J Cell Biol       Date:  1982-08       Impact factor: 10.539

10.  Direct demonstration of increased intracellular concentration of free calcium as measured by quin-2 in stimulated rat peritoneal mast cell.

Authors:  J R White; T Ishizaka; K Ishizaka; R Sha'afi
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205
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  1 in total

1.  Emerging biomedical and advanced applications of time-resolved fluorescence spectroscopy.

Authors:  J R Lakowicz; P A Koen; H Szmacinski; I Gryczynski; J Kuśba
Journal:  J Fluoresc       Date:  1994-03       Impact factor: 2.217
  1 in total

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