HYPOTHESIS: We hypothesized that livers with biliary atresia (BA) are more susceptible to the harmful effects of a high-pressure CO(2) pneumoperitoneum (PP) than healthy livers. METHODS: A murine model of BA was used in this experiment. Mice were divided into 6 groups: (1) control Balb/c; (2) control Balb/c, CO(2)-PP; (3) control BA; (4) BA-sham; (5) BA, CO(2)-PP; and (6) BA, air-PP. Mice from groups 2, 5, and 6 underwent an 8-mm Hg-PP for 60 minutes. Liver samples were collected for histology, colorimetry, and flow cytometry analysis 18 to 24 hours after the procedure. Markers of apoptosis were investigated as indicators of acute cell damage. RESULTS: We observed a statistically significant higher rate of apoptosis in livers with BA exposed to a prolonged CO(2)-PP or air-PP compared with control groups. There were no significant differences between groups 1 and 2, or between groups 5 and 6. CONCLUSIONS: In this animal model, we have shown that livers with BA are more susceptible than healthy livers to injury by a prolonged PP. This injury was caused by both CO(2) and air-PP, implying that it is the direct result of pressure. These results may have implications for the success of minimally invasive Kasai procedures.
HYPOTHESIS: We hypothesized that livers with biliary atresia (BA) are more susceptible to the harmful effects of a high-pressure CO(2) pneumoperitoneum (PP) than healthy livers. METHODS: A murine model of BA was used in this experiment. Mice were divided into 6 groups: (1) control Balb/c; (2) control Balb/c, CO(2)-PP; (3) control BA; (4) BA-sham; (5) BA, CO(2)-PP; and (6) BA, air-PP. Mice from groups 2, 5, and 6 underwent an 8-mm Hg-PP for 60 minutes. Liver samples were collected for histology, colorimetry, and flow cytometry analysis 18 to 24 hours after the procedure. Markers of apoptosis were investigated as indicators of acute cell damage. RESULTS: We observed a statistically significant higher rate of apoptosis in livers with BA exposed to a prolonged CO(2)-PP or air-PP compared with control groups. There were no significant differences between groups 1 and 2, or between groups 5 and 6. CONCLUSIONS: In this animal model, we have shown that livers with BA are more susceptible than healthy livers to injury by a prolonged PP. This injury was caused by both CO(2) and air-PP, implying that it is the direct result of pressure. These results may have implications for the success of minimally invasive Kasai procedures.
Authors: Ma Lishuang; Chen Zhen; Qiao Guoliang; Zhang Zhen; Wang Chen; Li Long; Liu Shuli Journal: Pediatr Surg Int Date: 2015-01-28 Impact factor: 1.827
Authors: Kin Wai E Chan; Kim Hung Lee; Siu Yan B Tsui; Yuen Shan Wong; Kit Yi K Pang; Jennifer Wai Cheung Mou; Yuk Him Tam Journal: Pediatr Surg Int Date: 2012-09-18 Impact factor: 1.827
Authors: Kin Wai E Chan; Kim Hung Lee; Hei Yi V Wong; Siu Yan B Tsui; Yuen Shan Wong; Kit Yi K Pang; Jennifer Wai Cheung Mou; Yuk Him Tam Journal: Pediatr Surg Int Date: 2014-04-11 Impact factor: 1.827
Authors: David Eugenio Hinojosa-Gonzalez; Luis C Bueno; Andres Roblesgil-Medrano; Gustavo Salgado-Garza; Sofia Hurtado-Arellano; Juan S Farias; Mauricio Torres-Martinez; Jaime A Escarcega-Bordagaray; Marcelo Salan-Gomez; Eduardo Flores-Villalba Journal: Pediatr Surg Int Date: 2021-07-16 Impact factor: 1.827