PURPOSE: The aim was to study transdermal electroporation of insulin-loaded nanocarriers as a methodology for delivering macromolecules. METHODS: The efficacy of electroporation of insulin as solution and nanoparticles was compared in vitro and in vivo. Histology and confocal laser scanning microscopy were used to assess the effects of electroporation on skin structure, whereas the latter also demonstrated the depth of permeation of the nanoparticles. In vivo studies were performed on streptozotocin-diabetic male Wistar rats and compared with subcutaneous administration. RESULTS: A linear increase in insulin flux was noted on increasing the applied voltage (R(2) = 0.9514), the number of pulses (R(2) = 0.8515), and the pulse length (R(2) = 0.9937). Electroporation of nanoparticles resulted in fourfold enhancement in insulin deposition in rat skin in contrast to solution. In vivo studies showed maximum reduction of 77 ± 5% (87.2 ± 6.4 mIU/mL, t = 2 hours) and 85 ± 8% (37.8 ± 10.2 mIU/mL, t = 4 hours) in blood glucose levels for solution and nanoparticles, respectively, with therapeutic levels maintained for 24 and 36 hours. CONCLUSION: Overall, electroporation of polymeric nanosystems proved to be an ideal alternative to injectable administration.
PURPOSE: The aim was to study transdermal electroporation of insulin-loaded nanocarriers as a methodology for delivering macromolecules. METHODS: The efficacy of electroporation of insulin as solution and nanoparticles was compared in vitro and in vivo. Histology and confocal laser scanning microscopy were used to assess the effects of electroporation on skin structure, whereas the latter also demonstrated the depth of permeation of the nanoparticles. In vivo studies were performed on streptozotocin-diabetic male Wistar rats and compared with subcutaneous administration. RESULTS: A linear increase in insulin flux was noted on increasing the applied voltage (R(2) = 0.9514), the number of pulses (R(2) = 0.8515), and the pulse length (R(2) = 0.9937). Electroporation of nanoparticles resulted in fourfold enhancement in insulin deposition in rat skin in contrast to solution. In vivo studies showed maximum reduction of 77 ± 5% (87.2 ± 6.4 mIU/mL, t = 2 hours) and 85 ± 8% (37.8 ± 10.2 mIU/mL, t = 4 hours) in blood glucose levels for solution and nanoparticles, respectively, with therapeutic levels maintained for 24 and 36 hours. CONCLUSION: Overall, electroporation of polymeric nanosystems proved to be an ideal alternative to injectable administration.
Authors: Yuqi Zhang; Jicheng Yu; Anna R Kahkoska; Jinqiang Wang; John B Buse; Zhen Gu Journal: Adv Drug Deliv Rev Date: 2018-12-08 Impact factor: 15.470
Authors: Abdul Ahad; Mohammad Raish; Yousef A Bin Jardan; Abdullah M Al-Mohizea; Fahad I Al-Jenoobi Journal: Pharmaceutics Date: 2021-01-14 Impact factor: 6.321