OBJECTIVES AND HYPOTHESIS: To determine the feasibility of using a multiple flow offline fractional exhaled nitric oxide (FeNO) collection method in an inner-city cohort and determine this population's alveolar and conducting airway contributions of NO. We hypothesized that the flow independent NO parameters would be associated differentially with wheeze and seroatopy. METHODS: As part of a birth cohort study, 9-year-old children (n=102) of African-American and Dominican mothers living in low-income NYC neighborhoods had FeNO samples collected offline at constant flow rates of 50, 83, and 100 ml/sec. Seroatopy was defined as having measurable (≥ 0.35 IU/ml) specific IgE to any of the five inhalant indoor allergens tested. Current wheeze (last 12 months) was assessed by ISAAC questionnaire. Bronchial NO flux (J(NO) ) and alveolar NO concentration (C(alv)) were estimated by the Pietropaoli and Hogman methods. RESULTS: Valid exhalation flow rates were achieved in 96% of the children. Children with seroatopy (53%) had significantly higher median J(NO) (522 pl/sec vs. 161 pl/sec, P<0.001) when compared to non-seroatopic children; however, median C(alv) was not significantly different between these two groups (5.5 vs. 5.8, P=0.644). Children with wheeze in the past year (21.6%) had significantly higher median C(alv) (8.4 ppb vs. 4.9 ppb, P<0.001), but not J(NO) (295 pl/sec vs. 165 pl/sec, P=0.241) when compared with children without wheeze. These associations remained stable after adjustment for known confounders/covariates. CONCLUSIONS: The multiple flow method was easily implemented in this pediatric inner-city cohort. In this study population, alveolar concentration of NO may be a better indicator of current wheeze than single flow FeNO.
OBJECTIVES AND HYPOTHESIS: To determine the feasibility of using a multiple flow offline fractional exhaled nitric oxide (FeNO) collection method in an inner-city cohort and determine this population's alveolar and conducting airway contributions of NO. We hypothesized that the flow independent NO parameters would be associated differentially with wheeze and seroatopy. METHODS: As part of a birth cohort study, 9-year-old children (n=102) of African-American and Dominican mothers living in low-income NYC neighborhoods had FeNO samples collected offline at constant flow rates of 50, 83, and 100 ml/sec. Seroatopy was defined as having measurable (≥ 0.35 IU/ml) specific IgE to any of the five inhalant indoor allergens tested. Current wheeze (last 12 months) was assessed by ISAAC questionnaire. Bronchial NO flux (J(NO) ) and alveolar NO concentration (C(alv)) were estimated by the Pietropaoli and Hogman methods. RESULTS: Valid exhalation flow rates were achieved in 96% of the children. Children with seroatopy (53%) had significantly higher median J(NO) (522 pl/sec vs. 161 pl/sec, P<0.001) when compared to non-seroatopic children; however, median C(alv) was not significantly different between these two groups (5.5 vs. 5.8, P=0.644). Children with wheeze in the past year (21.6%) had significantly higher median C(alv) (8.4 ppb vs. 4.9 ppb, P<0.001), but not J(NO) (295 pl/sec vs. 165 pl/sec, P=0.241) when compared with children without wheeze. These associations remained stable after adjustment for known confounders/covariates. CONCLUSIONS: The multiple flow method was easily implemented in this pediatric inner-city cohort. In this study population, alveolar concentration of NO may be a better indicator of current wheeze than single flow FeNO.
Authors: R L Miller; G L Chew; C A Bell; S A Biedermann; M Aggarwal; P L Kinney; W Y Tsai; R M Whyatt; F P Perera; J G Ford Journal: Am J Respir Crit Care Med Date: 2001-09-15 Impact factor: 21.405
Authors: S L Jones; P Herbison; J O Cowan; E M Flannery; R J Hancox; C R McLachlan; D R Taylor Journal: Eur Respir J Date: 2002-09 Impact factor: 16.671
Authors: Julie Kuriakose; Maria José Rosa; Matthew Perzanowski; Rachel Miller Journal: Am J Respir Crit Care Med Date: 2012-02-15 Impact factor: 21.405
Authors: Gregory E Miller; Edith Chen; Madeleine U Shalowitz; Rachel E Story; Adam K K Leigh; Paula Ham; Jesusa M G Arevalo; Steve W Cole Journal: Pediatr Pulmonol Date: 2018-03-12
Authors: Kathleen M Donohue; Rachel L Miller; Matthew S Perzanowski; Allan C Just; Lori A Hoepner; Srikesh Arunajadai; Stephen Canfield; David Resnick; Antonia M Calafat; Frederica P Perera; Robin M Whyatt Journal: J Allergy Clin Immunol Date: 2013-03 Impact factor: 10.793
Authors: Sandrah P Eckel; William S Linn; Kiros Berhane; Edward B Rappaport; Muhammad T Salam; Yue Zhang; Frank D Gilliland Journal: PLoS One Date: 2014-01-17 Impact factor: 3.240
Authors: Allan C Just; Robin M Whyatt; Rachel L Miller; Andrew G Rundle; Qixuan Chen; Antonia M Calafat; Adnan Divjan; Maria J Rosa; Hanjie Zhang; Frederica P Perera; Inge F Goldstein; Matthew S Perzanowski Journal: Am J Respir Crit Care Med Date: 2012-08-23 Impact factor: 21.405
Authors: Maria José Rosa; Matthew S Perzanowski; Adnan Divjan; Steven N Chillrud; Lori Hoepner; Hanjie Zhang; Robert Ridder; Frederica P Perera; Rachel L Miller Journal: Nitric Oxide Date: 2014-05-27 Impact factor: 4.427
Authors: Cristina Ardura-Garcia; Maritza Vaca; Gisela Oviedo; Carlos Sandoval; Lisa Workman; Alexander J Schuyler; Matthew S Perzanowski; Thomas A E Platts-Mills; Philip J Cooper Journal: Pediatr Allergy Immunol Date: 2015-08 Impact factor: 6.377
Authors: Philip J Cooper; Martha E Chico; Thomas Ae Platts-Mills; Laura C Rodrigues; David P Strachan; Mauricio L Barreto Journal: Int J Epidemiol Date: 2014-07-02 Impact factor: 7.196