BACKGROUND: Myocardial ischemia triggers the expression of multiple angiogenic factors including vascular endothelial growth factor and its receptors. However, vascular endothelial growth factor does not act in isolation. OBJECTIVE: To identify other genes important in the angiogenic response to clinically relevant myocardial ischemia. METHODS AND RESULTS: Paired intraoperative biopsies of ischemic and nonischemic myocardium were obtained from 12 patients with acute coronary syndromes (ACS) undergoing urgent coronary artery bypass graft surgery. Real-time polymerase chain reaction demonstrated significant upregulation of angiopoietin-2 (Ang-2) in ischemic myocardium, to a greater extent than other classical angiogenic factors. Microarray gene profiling identified Ang-2 to be among the top 10 differentially upregulated genes, in addition to genes involved in inflammation, cell signalling, remodelling and apoptosis. CONCLUSIONS: The present document is the first report of microarray analysis of patients with ACS, and supports an important role for Ang-2 in the angiogenic response to severe ischemia in the human heart. Common gene expression patterns in ACS may provide opportunities for targeted pharmacological and cellular intervention.
BACKGROUND:Myocardial ischemia triggers the expression of multiple angiogenic factors including vascular endothelial growth factor and its receptors. However, vascular endothelial growth factor does not act in isolation. OBJECTIVE: To identify other genes important in the angiogenic response to clinically relevant myocardial ischemia. METHODS AND RESULTS: Paired intraoperative biopsies of ischemic and nonischemic myocardium were obtained from 12 patients with acute coronary syndromes (ACS) undergoing urgent coronary artery bypass graft surgery. Real-time polymerase chain reaction demonstrated significant upregulation of angiopoietin-2 (Ang-2) in ischemic myocardium, to a greater extent than other classical angiogenic factors. Microarray gene profiling identified Ang-2 to be among the top 10 differentially upregulated genes, in addition to genes involved in inflammation, cell signalling, remodelling and apoptosis. CONCLUSIONS: The present document is the first report of microarray analysis of patients with ACS, and supports an important role for Ang-2 in the angiogenic response to severe ischemia in the human heart. Common gene expression patterns in ACS may provide opportunities for targeted pharmacological and cellular intervention.
Authors: Emma J Birks; Jennifer L Hall; Paul J R Barton; Suzanne Grindle; Najma Latif; James P Hardy; James E Rider; Nicholas R Banner; Asghar Khaghani; Leslie W Miller; Magdi H Yacoub Journal: Circulation Date: 2005-08-30 Impact factor: 29.690
Authors: Jan Kristian Damås; Torgun Waehre; Arne Yndestad; Kari Otterdal; Aina Hognestad; Nils Olav Solum; Lars Gullestad; Stig S Frøland; Pål Aukrust Journal: Circulation Date: 2003-05-12 Impact factor: 29.690
Authors: Aun Yeong Chong; Graham J Caine; Bethan Freestone; Andrew D Blann; Gregory Y H Lip Journal: J Am Coll Cardiol Date: 2004-02-04 Impact factor: 24.094
Authors: Reena Sandhu; Krystyna Teichert-Kuliszewska; Sukriti Nag; Gerald Proteau; Malcolm J Robb; Andrew I M Campbell; Michael A Kuliszewski; Michael J B Kutryk; Duncan J Stewart Journal: Cardiovasc Res Date: 2004-10-01 Impact factor: 10.787
Authors: Kathryn J Schunke; Chad B Walton; David R Veal; Chrisy T Mafnas; Cynthia D Anderson; Allison L Williams; Ralph V Shohet Journal: Cardiovasc Res Date: 2019-07-01 Impact factor: 10.787