| Literature DB >> 20846854 |
Xusheng Chen1, Lei Tang, Shu Li, Lijuan Liao, Jianhua Zhang, Zhonggui Mao.
Abstract
The goal of this study was to increase ε-Poly-L-Lysine (ε-PL) production by Streptomyces sp. M-Z18 through optimization of the culture medium. Glycerol, (NH4)2SO4 and K2HPO4 were the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design. The optimal concentration of the three factors was further investigated according to the central composite design and determined to be 59.48, 4.43 and -0.49 g/L, respectively. Under optimal conditions, a ε-PL concentration of 2.27±0.15 g/L was obtained. Compared with glucose, fermentation of glycerol afforded 2.8-fold and 6.3-fold the ε-PL titer and yield, respectively. Therefore, glycerol may be an efficient carbon source for ε-PL production by Streptomyces sp. M-Z18. Copyright ÂEntities:
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Year: 2010 PMID： 20846854 DOI： 10.1016/j.biortech.2010.08.071
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642