| Literature DB >> 20845374 |
Haibo Li1, Scharri J Ezell1, Xiangrong Zhang1, Wei Wang1, Hongxia Xu1,2, Elizabeth R Rayburn1, Xu Zhang1, Evrim Gurpinar1, Xinyi Yang1, Charnell I Sommers1, Sadanandan E Velu3,4, Ruiwen Zhang1,4.
Abstract
We herein describe the development and validation of an HPLC method for the quantitation of 7-(benzylamino)-1,3,4,8-tetrahydropyrrolo [4,3,2-de]quinolin-8(1H)-one (BA-TPQ), a newly synthesized iminoquinone anticancer agent. BA-TPQ was extracted from plasma and tissue samples by first precipitating proteins with acetonitrile followed by a liquid-liquid extraction with ethyl acetate. Chromatographic separation was carried out using a gradient flow rate on a Zorbax SB C(18) column, and the effluent was monitored by UV detection at 346 nm. The method was found to be precise, accurate, and specific, with a linear range of 3.91-1955.0 ng/mL in plasma, 19.55-1955.0 ng/mL in spleen, brain, and liver homogenates and 19.55-3910.0 ng/mL in heart, lung and kidney homogenates. The method was stable under all relevant conditions. Using this method, we also carried out an initial study determining plasma pharmacokinetics and tissue distribution of BA-TPQ in mice following intravenous administration. In summary, this simple and sensitive HPLC method can be used in future preclinical and clinical studies of BA-TPQ.Entities:
Mesh：
Substances：
Year: 2010 PMID： 20845374 PMCID： PMC3769168 DOI： 10.1002/bmc.1498
Source DB: PubMed Journal: Biomed Chromatogr ISSN： 0269-3879 Impact factor: 1.902