AIM: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. METHODS AND RESULTS: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negative isolates showed more antibiotic susceptibility than VR positive isolates. 30% of the isolates were positive for the VR and carried the genes dfrA7, dfrA17-aadA5, dfrA1-aadA1, dfrA12-orf5-aadA2 and bla(OXA-30)-aadA1. Five restriction patterns were detected and isolates with the same VR amplicon size had the same restriction pattern. CONCLUSIONS: Our data demonstrated that Class I integrons are widely disseminated in Lebanon and showed their importance for the occurrence and transmission of multidrug resistance. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in UPEC.
AIM: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. METHODS AND RESULTS: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negative isolates showed more antibiotic susceptibility than VR positive isolates. 30% of the isolates were positive for the VR and carried the genes dfrA7, dfrA17-aadA5, dfrA1-aadA1, dfrA12-orf5-aadA2 and bla(OXA-30)-aadA1. Five restriction patterns were detected and isolates with the same VR amplicon size had the same restriction pattern. CONCLUSIONS: Our data demonstrated that Class I integrons are widely disseminated in Lebanon and showed their importance for the occurrence and transmission of multidrug resistance. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in UPEC.
Authors: Amy S Labar; Jennifer S Millman; Ellen Ruebush; Japheth A Opintan; Rima A Bishar; A Oladipo Aboderin; Mercy J Newman; Adebayo Lamikanra; Iruka N Okeke Journal: PLoS One Date: 2012-05-30 Impact factor: 3.240
Authors: Sara A Ochoa; Ariadnna Cruz-Córdova; Victor M Luna-Pineda; Juan P Reyes-Grajeda; Vicenta Cázares-Domínguez; Gerardo Escalona; Ma Eugenia Sepúlveda-González; Fernanda López-Montiel; José Arellano-Galindo; Briceida López-Martínez; Israel Parra-Ortega; Silvia Giono-Cerezo; Rigoberto Hernández-Castro; Daniela de la Rosa-Zamboni; Juan Xicohtencatl-Cortes Journal: Front Microbiol Date: 2016-12-21 Impact factor: 5.640