Literature DB >> 20830338

Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching.

Tchern Lenn1, Christos N Gkekas, Laurent Bernard, Christoph Engl, Goran Jovanovic, Martin Buck, Liming Ying.   

Abstract

We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.

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Year:  2010        PMID： 20830338      PMCID： PMC3337751          DOI： 10.1039/c0cc01707h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  19 in total

1.  Confocal FRET microscopy to measure clustering of ligand-receptor complexes in endocytic membranes.

Authors:  Horst Wallrabe; Masilamani Elangovan; Almut Burchard; Ammasi Periasamy; Margarida Barroso
Journal:  Biophys J       Date:  2003-07       Impact factor: 4.033

2.  The vesicle-inducing protein 1 from Synechocystis sp. PCC 6803 organizes into diverse higher-ordered ring structures.

Authors:  Eva Fuhrmann; Jelle B Bultema; Uwe Kahmann; Eva Rupprecht; Egbert J Boekema; Dirk Schneider
Journal:  Mol Biol Cell       Date:  2009-09-23       Impact factor: 4.138

3.  Heterospecies partition analysis reveals binding curve and stoichiometry of protein interactions in living cells.

Authors:  Bin Wu; Yan Chen; Joachim D Müller
Journal:  Proc Natl Acad Sci U S A       Date:  2010-02-08       Impact factor: 11.205

4.  Molecular determinants for PspA-mediated repression of the AAA transcriptional activator PspF.

Authors:  Sarah Elderkin; Patricia Bordes; Susan Jones; Mathieu Rappas; Martin Buck
Journal:  J Bacteriol       Date:  2005-05       Impact factor: 3.490

Review 5.  Super-resolution fluorescence microscopy.

Authors:  Bo Huang; Mark Bates; Xiaowei Zhuang
Journal:  Annu Rev Biochem       Date:  2009       Impact factor: 23.643

6.  Clustering and dynamics of cytochrome bd-I complexes in the Escherichia coli plasma membrane in vivo.

Authors:  Tchern Lenn; Mark C Leake; Conrad W Mullineaux
Journal:  Mol Microbiol       Date:  2008-10-23       Impact factor: 3.501

7.  Stoichiometry of molecular complexes at adhesions in living cells.

Authors:  Michelle A Digman; Paul W Wiseman; Colin Choi; Alan R Horwitz; Enrico Gratton
Journal:  Proc Natl Acad Sci U S A       Date:  2009-01-23       Impact factor: 11.205

8.  Variable stoichiometry of the TatA component of the twin-arginine protein transport system observed by in vivo single-molecule imaging.

Authors:  Mark C Leake; Nicholas P Greene; Rachel M Godun; Thierry Granjon; Grant Buchanan; Shuyun Chen; Richard M Berry; Tracy Palmer; Ben C Berks
Journal:  Proc Natl Acad Sci U S A       Date:  2008-10-01       Impact factor: 11.205

9.  In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli.

Authors:  Christoph Engl; Goran Jovanovic; Louise J Lloyd; Heath Murray; Martin Spitaler; Liming Ying; Jeff Errington; Martin Buck
Journal:  Mol Microbiol       Date:  2009-06-22       Impact factor: 3.501

10.  A lower-order oligomer form of phage shock protein A (PspA) stably associates with the hexameric AAA(+) transcription activator protein PspF for negative regulation.

Authors:  Nicolas Joly; Patricia C Burrows; Christoph Engl; Goran Jovanovic; Martin Buck
Journal:  J Mol Biol       Date:  2009-10-03       Impact factor: 5.469
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  8 in total

1.  Single molecule fluorescence study of the Bacillus thuringiensis toxin Cry1Aa reveals tetramerization.

Authors:  Nicolas Groulx; Hugo McGuire; Raynald Laprade; Jean-Louis Schwartz; Rikard Blunck
Journal:  J Biol Chem       Date:  2011-10-17       Impact factor: 5.157

Review 2.  Structural disorder in plant proteins: where plasticity meets sessility.

Authors:  Alejandra A Covarrubias; Cesar L Cuevas-Velazquez; Paulette S Romero-Pérez; David F Rendón-Luna; Caspar C C Chater
Journal:  Cell Mol Life Sci       Date:  2017-06-22       Impact factor: 9.261

3.  Correction of Systematic Bias in Single Molecule Photobleaching Measurements.

Authors:  Simli Dey; Anirban Das; Sudipta Maiti
Journal:  Biophys J       Date:  2020-01-11       Impact factor: 4.033

4.  Interactions and Localization of Escherichia coli Error-Prone DNA Polymerase IV after DNA Damage.

Authors:  Sarita Mallik; Ellen M Popodi; Andrew J Hanson; Patricia L Foster
Journal:  J Bacteriol       Date:  2015-06-22       Impact factor: 3.490

5.  Changes in Psp protein binding partners, localization and behaviour upon activation of the Yersinia enterocolitica phage shock protein response.

Authors:  Saori Yamaguchi; Dylan A Reid; Eli Rothenberg; Andrew J Darwin
Journal:  Mol Microbiol       Date:  2013-01-07       Impact factor: 3.501

Review 6.  Functioning nanomachines seen in real-time in living bacteria using single-molecule and super-resolution fluorescence imaging.

Authors:  Sheng-Wen Chiu; Mark C Leake
Journal:  Int J Mol Sci       Date:  2011-04-15       Impact factor: 5.923

Review 7.  Experimental approaches for addressing fundamental biological questions in living, functioning cells with single molecule precision.

Authors:  Tchern Lenn; Mark C Leake
Journal:  Open Biol       Date:  2012-06       Impact factor: 6.411

8.  Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells.

Authors:  Parul Mehta; Goran Jovanovic; Tchern Lenn; Andreas Bruckbauer; Christoph Engl; Liming Ying; Martin Buck
Journal:  Nat Commun       Date:  2013       Impact factor: 14.919
  8 in total

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