Literature DB >> 20828533

A reliable method to concentrate circulating DNA.

Olga Bryzgunova1, Anna Bondar, Evgeniy Morozkin, Vladislav Mileyko, Valentin Vlassov, Pavel Laktionov.   

Abstract

Concentration of circulating DNA probes is required to increase the amount of DNA involved in subsequent study (by polymerase chain reaction, sequencing, and microarray). This work was dedicated to the comparison of five different methods used for concentration of DNA circulating in blood. Precipitation of circulating DNA with acetone in the presence of triethylamine provides minimal DNA loss, high reproducibility, and at least three times higher DNA yield in comparison with the standard ethanol protocol.
Copyright © 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20828533     DOI： 10.1016/j.ab.2010.09.005

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Comparative Study of Extracellular Vesicles from the Urine of Healthy Individuals and Prostate Cancer Patients.

Authors:  Olga E Bryzgunova; Marat M Zaripov; Tatyana E Skvortsova; Evgeny A Lekchnov; Alina E Grigor'eva; Ivan A Zaporozhchenko; Evgeny S Morozkin; Elena I Ryabchikova; Yuri B Yurchenko; Vladimir E Voitsitskiy; Pavel P Laktionov
Journal:  PLoS One       Date:  2016-06-15       Impact factor: 3.240

2.  Blood Plasma Exosomes Contain Circulating DNA in Their Crown.

Authors:  Oleg Tutanov; Tatiana Shtam; Alina Grigor'eva; Alexey Tupikin; Yuri Tsentalovich; Svetlana Tamkovich
Journal:  Diagnostics (Basel)       Date:  2022-03-30

3.  DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect.

Authors:  Remigiusz K Trojanowicz; Ambra Vestri; Massimo Rippa; Joseph Zyss; Katarzyna Matczyszyn; Lucia Petti
Journal:  ACS Omega       Date:  2022-08-30

4.  Size and Methylation Index of Cell-Free and Cell-Surface-Bound DNA in Blood of Breast Cancer Patients in the Contest of Liquid Biopsy.

Authors:  Svetlana Tamkovich; Alexey Tupikin; Anton Kozyakov; Pavel Laktionov
Journal:  Int J Mol Sci       Date:  2022-08-10       Impact factor: 6.208

Review 5.  Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods.

Authors:  Olga Bryzgunova; Maria Konoshenko; Ivan Zaporozhchenko; Alexey Yakovlev; Pavel Laktionov
Journal:  Diagnostics (Basel)       Date:  2021-05-11

6.  Locus-Specific Methylation of GSTP1, RNF219, and KIAA1539 Genes with Single Molecule Resolution in Cell-Free DNA from Healthy Donors and Prostate Tumor Patients: Application in Diagnostics.

Authors:  Olga Bryzgunova; Anna Bondar; Pavel Ruzankin; Petr Laktionov; Anton Tarasenko; Alexander Kurilshikov; Rostislav Epifanov; Marat Zaripov; Marsel Kabilov; Pavel Laktionov
Journal:  Cancers (Basel)       Date:  2021-12-12       Impact factor: 6.639
  6 in total

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