Qi Li1, Bao Zhang. 1. Department of Otolaryngology-Head and Neck Surgery, and Bao Zhang: Molecular Biology Laboratory, Southern Medical University, Guangzhou, Guangdong, PR China.
Abstract
OBJECTIVE: To investigate the expression of aquaporin-1 (AQP1) in nasopharyngeal tumour and nontumour tissues. METHOD: Thirty nasopharyngeal biopsy samples were collected and examined with both immunohistochemistry and real-time fluorescence quantitative polymerase chain reaction (RT-PCR) to detect the expression level of AQP1 in tissues. RESULTS: Immunohistochemistry data suggested that AQP1 was expressed in both nasopharyngeal tumour and normal tissues, but tumour tissues were stained a lot stronger than nontumour tissues. RT-PCR analysis further confirmed the overexpression of AQP1 in tumour tissues; migrated tumour tissue had even higher expression. CONCLUSION: The expression level of aquaporin-1 in nasopharyngeal cancer tissue could be involved in tumour migration.
OBJECTIVE: To investigate the expression of aquaporin-1 (AQP1) in nasopharyngeal tumour and nontumour tissues. METHOD: Thirty nasopharyngeal biopsy samples were collected and examined with both immunohistochemistry and real-time fluorescence quantitative polymerase chain reaction (RT-PCR) to detect the expression level of AQP1 in tissues. RESULTS: Immunohistochemistry data suggested that AQP1 was expressed in both nasopharyngeal tumour and normal tissues, but tumour tissues were stained a lot stronger than nontumour tissues. RT-PCR analysis further confirmed the overexpression of AQP1 in tumour tissues; migrated tumour tissue had even higher expression. CONCLUSION: The expression level of aquaporin-1 in nasopharyngeal cancer tissue could be involved in tumour migration.
Authors: Yoko Tomita; Hilary Dorward; Andrea J Yool; Eric Smith; Amanda R Townsend; Timothy J Price; Jennifer E Hardingham Journal: Int J Mol Sci Date: 2017-01-29 Impact factor: 5.923