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Literature DB >> 2082815

Immunochemical analysis of the carbohydrate moiety of yeast killer toxin K28.

Abstract

Killer toxin K28, a 16 kd protein secreted by the wine yeast Saccharomyces cerevisiae strain 28, was reversibly bound by a column of Concanavalin A-Sepharose, confirming its glycoprotein nature. HPLC analysis of acid hydrolyzates of K28 toxin as well as Western-blots of beta-eliminated and/or endo H-treated killer toxin preparations probed with polyclonal alpha-toxin antibodies revealed that the carbohydrate moiety of K28 consists of D-mannose only, which is O-glycosidically linked via Ser/Thr residues to the protein part. The change in gel mobility of K28 after beta-elimination was caused by a decrease in molecular mass of about 1,800, corresponding to a carbohydrate moiety of 10 mannose residues per killer toxin molecule.

Entities: Chemical Disease Species

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Year: 1990 PMID： 2082815 DOI： 10.1007/BF00399340

Source DB: PubMed Journal: Antonie Van Leeuwenhoek ISSN： 0003-6072 Impact factor: 2.271

14 in total

1. Mannoprotein of the yeast cell wall as primary receptor for the killer toxin of Saccharomyces cerevisiae strain 28.

Authors: M Schmitt; F Radler
Journal: J Gen Microbiol Date: 1987-12

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

3. Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes.

Authors: R Palacios; R D Palmiter; R T Schimke
Journal: J Biol Chem Date: 1972-04-25 Impact factor: 5.157

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

Review 5. Double-stranded RNA replication in yeast: the killer system.

Authors: R B Wickner
Journal: Annu Rev Biochem Date: 1986 Impact factor: 23.643

6. Sequence of the preprotoxin dsRNA gene of type I killer yeast: multiple processing events produce a two-component toxin.

Authors: K A Bostian; Q Elliott; H Bussey; V Burn; A Smith; D J Tipper
Journal: Cell Date: 1984-03 Impact factor: 41.582

Immunochemical analysis of the carbohydrate moiety of yeast killer toxin K28.

1. Mannoprotein of the yeast cell wall as primary receptor for the killer toxin of Saccharomyces cerevisiae strain 28.

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

3. Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Review 5. Double-stranded RNA replication in yeast: the killer system.

6. Sequence of the preprotoxin dsRNA gene of type I killer yeast: multiple processing events produce a two-component toxin.

7. Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2.

8. The structure of a beta-(1 leads to 3)-D-glucan from yeast cell walls.

9. Effect of yeast killer toxin on sensitive cells of Saccharomyces cerevisiae.

10. Cell wall receptor for yeast killer toxin: involvement of (1 leads to 6)-beta-D-glucan.

1. Beta-1,3-glucanase from Delftia tsuruhatensis strain MV01 and its potential application in vinification.