| Literature DB >> 20826932 |
M A Vikhrova1, M V Shveygert, E A Khrapov, M L Filipenko, I P Gileva, V V Morozova, N V Tikunova.
Abstract
Four unique phage single-chain variable fragments (scFvs) to recombinant human interleukin 18 (IL-18) has been selected from a naïve combinatorial library of human scFvs. Binding of unique phage antibodies with IL-18 was tested by ELISA and Western-blotting. No cross reactivity with tumor necrosis factor α, interferons α and γ was shown for the selected antibodies. The gene segments encoding V(D)J regions of selected antibodies exhibited a high degree of homology to germline genes, therefore we suggest that the selected scFv's belong to repertoire of naïve autoantibodies.Entities:
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Year: 2010 PMID： 20826932 DOI： 10.3233/HAB-2010-0230
Source DB: PubMed Journal: Hum Antibodies ISSN： 1093-2607