Yiliang Zhang1, Ru Zhang, Chunfu Dai, Peter S Steyger, Yongfu Yu. 1. Department of Thoracic Surgery, Fudan University Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.
Abstract
OBJECTIVE/HYPOTHESIS: The distribution of gentamicin in the inner ear via the endolymphatic sac (ES) or round window (RW) routes was investigated. STUDY DESIGN: Experimental study. METHODS: A fluorescent gentamicin-Texas Red conjugate (GTTR) was adopted to visualize the gentamicin. Adult guinea pigs were treated with GTTR applied to the ES or RW, the animals were allowed to recover for 3 days, then confocal microscopy was used to observe and quantify GTTR distributions in cochlear and vestibular sensory epithelium. RESULTS: When GTTR was applied via the ES, strong GTTR labeling was observed in the vestibule while little GTTR was detected in the cochlea (P < .0001). However, distinct GTTR fluorescence was observed in the cochlea and vestibule following RW application (P = .7967). There was less GTTR labeling in cochlea via ES application than through RW administration (P < .0001). CONCLUSIONS: ES drug application may be preferable for the treatment of intractable Meniere's disease. Laryngoscope, 2010.
OBJECTIVE/HYPOTHESIS: The distribution of gentamicin in the inner ear via the endolymphatic sac (ES) or round window (RW) routes was investigated. STUDY DESIGN: Experimental study. METHODS: A fluorescent gentamicin-Texas Red conjugate (GTTR) was adopted to visualize the gentamicin. Adult guinea pigs were treated with GTTR applied to the ES or RW, the animals were allowed to recover for 3 days, then confocal microscopy was used to observe and quantify GTTR distributions in cochlear and vestibular sensory epithelium. RESULTS: When GTTR was applied via the ES, strong GTTR labeling was observed in the vestibule while little GTTR was detected in the cochlea (P < .0001). However, distinct GTTR fluorescence was observed in the cochlea and vestibule following RW application (P = .7967). There was less GTTR labeling in cochlea via ES application than through RW administration (P < .0001). CONCLUSIONS: ES drug application may be preferable for the treatment of intractable Meniere's disease. Laryngoscope, 2010.
Authors: Gordon H Sun; Randal Leung; Ravi N Samy; Jacob S McAfee; Matthew J Hearst; Christopher R Savage; Daniel I Choo; Myles L Pensak Journal: Laryngoscope Date: 2010-03 Impact factor: 3.325
Authors: Jian-Ping Liu; Chun-Fu Dai; Zheng-Min Wang; Fang-Lu Chi; Jie Tian; Cui-Di Da Journal: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi Date: 2006-11