Liquid chromatography with amperometric detection for the determination of non-steroidal anti-inflammatory drugs in plasma.

A simple and sensitive liquid chromatographic method with electrochemical detection is described for the quantitative determination of the non-steroidal anti-inflammatory drugs diflunisal, indomethacin, naproxen, piroxicam and sulindac in human plasma. Isolation of the drug from the biological fluid is achieved using a Sep-pak RP18 cartridge. Separation of plasma components occurs on a reversed-phase C18 column with a mobile phase consisting of methanol-water-phosphate buffer. For the amperometric detection the potential of +0.9 V was set on the working electrode. The detection limit of the assay is 10-20 ng/ml. The method showed good concordance for plasma samples containing the drugs (r = 0.999) and can be readily utilized for clinical pharmacokinetic studies.