Manipulation and isolation of single cells and nuclei.

The heterogeneous behavior of cells within a cell population makes measurements at the multicellular level insensitive to changes in single cells. Single-cell and single-nucleus analyses are therefore important to address this deficiency which will aid in the understanding of fundamental biology at both the cellular and subcellular levels. Recent technological advancements have enabled the development of new methodologies capable of handling these new challenges. This review highlights various techniques used in single-cell and single-nucleus manipulation and isolation. In particular, the applications related to microfluidics, electrical, optical, and physical methods will be discussed. Ultimately, it is hoped that these techniques will enable fundamental tests to be conducted on single cells and nuclei. One important potential outcome is that this will contribute not only towards detection and isolation of diseased cells but also more accurate diagnosis and prognosis of human diseases.