Literature DB >> 20816160

Transcript analysis of stem cells.

Alison V Nairn1, Mitche dela Rosa, Kelley W Moremen.   

Abstract

Quantitative real-time polymerase chain reaction (qRT-PCR) is a flexible and scalable method for analyzing transcript abundance that can be used at a single gene or high-throughput (>100 genes) level. Information obtained from this technique can be used as an indicator of potential regulation of glycosylation at the transcript level when combined with glycan structural or protein abundance data. This chapter describes detailed methods to design and perform qRT-PCR analyses and provides examples of information that can be obtained from the technique. Copyright (c) 2010 Elsevier Inc. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20816160      PMCID： PMC3104320          DOI： 10.1016/S0076-6879(10)79004-2

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  52 in total

1.  KEGG: kyoto encyclopedia of genes and genomes.

Authors:  M Kanehisa; S Goto
Journal:  Nucleic Acids Res       Date:  2000-01-01       Impact factor: 16.971

Review 2.  Glycosylation, immunity, and autoimmunity.

Authors:  J B Lowe
Journal:  Cell       Date:  2001-03-23       Impact factor: 41.582

Review 3.  Essential roles of carbohydrate signals in development, immune response and tissue functions, as revealed by gene targeting.

Authors:  T Muramatsu
Journal:  J Biochem       Date:  2000-02       Impact factor: 3.387

4.  Primer3 on the WWW for general users and for biologist programmers.

Authors:  S Rozen; H Skaletsky
Journal:  Methods Mol Biol       Date:  2000

5.  FUT4 and FUT9 genes are expressed early in human embryogenesis.

Authors:  A Cailleau-Thomas; P Coullin; J J Candelier; L Balanzino; B Mennesson; R Oriol; R Mollicone
Journal:  Glycobiology       Date:  2000-08       Impact factor: 4.313

Review 6.  Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming.

Authors:  Rudolf Jaenisch; Richard Young
Journal:  Cell       Date:  2008-02-22       Impact factor: 41.582

Review 7.  The joys of HexNAc. The synthesis and function of N- and O-glycan branches.

Authors:  H Schachter
Journal:  Glycoconj J       Date:  2000 Jul-Sep       Impact factor: 2.916

8.  Regulation of glycan structures in animal tissues: transcript profiling of glycan-related genes.

Authors:  Alison V Nairn; William S York; Kyle Harris; Erica M Hall; J Michael Pierce; Kelley W Moremen
Journal:  J Biol Chem       Date:  2008-04-14       Impact factor: 5.157

9.  Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies.

Authors:  Hyejung Park; Christopher A Haynes; Alison V Nairn; Michael Kulik; Stephen Dalton; Kelley Moremen; Alfred H Merrill
Journal:  J Lipid Res       Date:  2009-09-28       Impact factor: 5.922

10.  KEGG for linking genomes to life and the environment.

Authors:  Minoru Kanehisa; Michihiro Araki; Susumu Goto; Masahiro Hattori; Mika Hirakawa; Masumi Itoh; Toshiaki Katayama; Shuichi Kawashima; Shujiro Okuda; Toshiaki Tokimatsu; Yoshihiro Yamanishi
Journal:  Nucleic Acids Res       Date:  2007-12-12       Impact factor: 16.971
View more
  17 in total

Review 1.  Vertebrate protein glycosylation: diversity, synthesis and function.

Authors:  Kelley W Moremen; Michael Tiemeyer; Alison V Nairn
Journal:  Nat Rev Mol Cell Biol       Date:  2012-06-22       Impact factor: 94.444

2.  Sialylation regulates brain structure and function.

Authors:  Seung-Wan Yoo; Mary G Motari; Keiichiro Susuki; Jillian Prendergast; Andrea Mountney; Andres Hurtado; Ronald L Schnaar
Journal:  FASEB J       Date:  2015-04-06       Impact factor: 5.191

3.  O-Linked N-Acetylglucosamine (O-GlcNAc) Expression Levels Epigenetically Regulate Colon Cancer Tumorigenesis by Affecting the Cancer Stem Cell Compartment via Modulating Expression of Transcriptional Factor MYBL1.

Authors:  Huabei Guo; Bing Zhang; Alison V Nairn; Tamas Nagy; Kelley W Moremen; Phillip Buckhaults; Michael Pierce
Journal:  J Biol Chem       Date:  2017-01-17       Impact factor: 5.157

4.  Helicobacter pylori chronic infection and mucosal inflammation switches the human gastric glycosylation pathways.

Authors:  Ana Magalhães; Ricardo Marcos-Pinto; Alison V Nairn; Mitche Dela Rosa; Rui M Ferreira; Susana Junqueira-Neto; Daniela Freitas; Joana Gomes; Patrícia Oliveira; Marta R Santos; Nuno T Marcos; Wen Xiaogang; Céu Figueiredo; Carla Oliveira; Mário Dinis-Ribeiro; Fátima Carneiro; Kelley W Moremen; Leonor David; Celso A Reis
Journal:  Biochim Biophys Acta       Date:  2015-07-02

5.  Isotopic labeling with cellular O-glycome reporter/amplification (ICORA) for comparative O-glycomics of cultured cells.

Authors:  Matthew R Kudelka; Alison V Nairn; Mohammed Y Sardar; Xiaodong Sun; Elliot L Chaikof; Tongzhong Ju; Kelley W Moremen; Richard D Cummings
Journal:  Glycobiology       Date:  2018-04-01       Impact factor: 4.313

6.  Metabolic glycoengineering of mesenchymal stromal cells with N-propanoylmannosamine.

Authors:  Suvi Natunen; Milla Lampinen; Heli Suila; Ilja Ritamo; Virve Pitkänen; Alison V Nairn; Jarkko Räbinä; Saara Laitinen; Kelley W Moremen; Werner Reutter; Leena Valmu
Journal:  Glycobiology       Date:  2013-05-25       Impact factor: 4.313

7.  Identification of potential glycan cancer markers with sialic acid attached to sialic acid and up-regulated fucosylated galactose structures in epidermal growth factor receptor secreted from A431 cell line.

Authors:  Shiaw-Lin Wu; Allen D Taylor; Qiaozhen Lu; Samir M Hanash; Hogune Im; Michael Snyder; William S Hancock
Journal:  Mol Cell Proteomics       Date:  2013-01-31       Impact factor: 5.911

8.  O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signaling.

Authors:  Nithya Selvan; Stephan George; Fatema J Serajee; Marie Shaw; Lynne Hobson; Vera Kalscheuer; Nripesh Prasad; Shawn E Levy; Juliet Taylor; Salim Aftimos; Charles E Schwartz; Ahm M Huq; Jozef Gecz; Lance Wells
Journal:  J Biol Chem       Date:  2018-05-16       Impact factor: 5.157

9.  Regulation of glycan structures in murine embryonic stem cells: combined transcript profiling of glycan-related genes and glycan structural analysis.

Authors:  Alison V Nairn; Kazuhiro Aoki; Mitche dela Rosa; Mindy Porterfield; Jae-Min Lim; Michael Kulik; J Michael Pierce; Lance Wells; Stephen Dalton; Michael Tiemeyer; Kelley W Moremen
Journal:  J Biol Chem       Date:  2012-09-17       Impact factor: 5.157

10.  RElative QUantitation Inferred by Evaluating Mixtures (REQUIEM).

Authors:  Sami T Tuomivaara; Paul Schliekelman; Alison V Nairn; Kelley W Moremen; William S York
Journal:  Anal Chim Acta       Date:  2017-10-04       Impact factor: 6.558
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.