Literature DB >> 20814929

Application of dynamic light scattering in protein crystallization.

Ariane Proteau1, Rong Shi, Miroslaw Cygler.   

Abstract

Success in determining the three-dimensional structure of a macromolecule by X-ray diffraction methods depends critically on the ability to obtain well ordered crystals of the macromolecule in question. Predisposition to crystallization correlates with the homogeneity of the molecules in solution. Dynamic light scattering (DLS) is particularly well suited for evaluating protein homogeneity under multiple conditions and at concentrations commensurate with crystallization conditions. This unit presents a typical protocol for DLS measurements of a protein sample, and describes approaches to improve protein homogeneity in solution.

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Year:  2010        PMID： 20814929     DOI： 10.1002/0471140864.ps1710s61

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  7 in total

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Review 5.  Serial femtosecond crystallography: A revolution in structural biology.

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6.  Biophysical Characterization of a Vaccine Candidate against HIV-1: The Transmembrane and Membrane Proximal Domains of HIV-1 gp41 as a Maltose Binding Protein Fusion.

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Journal:  PLoS One       Date:  2015-08-21       Impact factor: 3.240

7.  Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1.

Authors:  Ho-Hsien Lee; Irene Cherni; HongQi Yu; Raimund Fromme; Jeffrey D Doran; Ingo Grotjohann; Michele Mittman; Shibom Basu; Arpan Deb; Katerina Dörner; Andrew Aquila; Anton Barty; Sébastien Boutet; Henry N Chapman; R Bruce Doak; Mark S Hunter; Daniel James; Richard A Kirian; Christopher Kupitz; Robert M Lawrence; Haiguang Liu; Karol Nass; Ilme Schlichting; Kevin E Schmidt; M Marvin Seibert; Robert L Shoeman; John C H Spence; Francesco Stellato; Uwe Weierstall; Garth J Williams; Chunhong Yoon; Dingjie Wang; Nadia A Zatsepin; Brenda G Hogue; Nobuyuki Matoba; Petra Fromme; Tsafrir S Mor
Journal:  IUCrJ       Date:  2014-08-20       Impact factor: 4.769
  7 in total

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