| Literature DB >> 20807554 |
Athina Avlami1, S Bekris, G Ganteris, Eleni Kraniotaki, Eleni Malamou-Lada, Maria Orfanidou, Olga Paniara, Angeliki Pantazatou, C C Papagiannitsis, Evangelia Platsouka, Ioanna Stefanou, Eva Tzelepi, Helen Vagiakou, Vivi Miriagou.
Abstract
hyplex®-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-β-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensitivity (98.0%) and specificity (98.6%) and was proven reliable in detecting bla(VIM) genes in blood, urine, pus, and sputum samples that, as confirmed by conventional methods, contained various VIM-producing species. Future multicenter studies should be considered for the thorough evaluation of this method and its potential diagnostic utility.Entities:
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Year: 2010 PMID： 20807554 DOI： 10.1016/j.mimet.2010.08.014
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363