Temporal and illumination-induced variations in the in vivo light transmission spectra of four photosensitizers in EMT6/Ed murine tumours.

Temporal and illumination-induced variations in the in vivo light transmission spectrum of the photosensitizer will influence light dosimetry for photodynamic therapy (PDT). The present authors have studied the in vivo spectra of four photosensitizers in the EMT6/Ed murine tumour model in Balb/c mice. The following photosensitizers were used: bis(dimethylthexylsiloxy)silicon 2,3-naphthalocyanine (SiNc 8), benzoporphyrin-derivative monoacid ring A (BPD Verteporfin), Photofrin and ethanolamined hypocrellin B (HBEA-R2). Spectra were measured non-invasively in the EMT6/Ed murine tumour model in the spectral range 600-840 nm, using a diode laser, a dye laser and a Ti:sapphire laser. Red-shift and broadening of the SiNc 8 absorption band was observed at 790 nm, and a slight red-shift was observed in the BPD, HBEA-R2 and Photofrin in vivo absorption spectrum. Exposure to 300 J of light at the peak absorption wavelength caused complete photobleaching of BPD at 690 nm, and a reduced absorption by SiNc 8 at 780 nm, Photofrin at 626 nm, and HBEA-R2 at 656 nm.