BACKGROUND AND OBJECTIVES: To determine whether bioluminescence imaging of human lung cancer cells growing in an orthotopic murine model provides a sensitive tool for monitoring tumor progression in athymic nude mice. METHODS: Human lung cancer (A549) cells were stably transfected with the firefly luciferase gene and inoculated into the right lung of athymic nude mice. Seven days after inoculation tumor growth was evaluated using the Kodak in-vivo Imaging System FX and continued to be monitored on a weekly basis. RESULTS: In duplicate experiments, human lung cancer tumors formed in 90% of animal's injected orthotopically. The mean intensity of the bioluminescence signal emitted from the lung cancer cells increased logarithmically during the course of study. Mice with positive bioluminescence signaling had confirmed tumors by microscopic histological analysis. Bioluminescence activity had a strong correlation with the tumor volume as determined histologically. CONCLUSIONS: Bioluminescence intensity directly correlates with tumor volume and therefore offers a reliable approach for detecting and monitoring the growth of human lung cancer cells in orthotopic murine models.
BACKGROUND AND OBJECTIVES: To determine whether bioluminescence imaging of humanlung cancer cells growing in an orthotopic murine model provides a sensitive tool for monitoring tumor progression in athymic nude mice. METHODS:Humanlung cancer (A549) cells were stably transfected with the firefly luciferase gene and inoculated into the right lung of athymic nude mice. Seven days after inoculation tumor growth was evaluated using the Kodak in-vivo Imaging System FX and continued to be monitored on a weekly basis. RESULTS: In duplicate experiments, humanlung cancer tumors formed in 90% of animal's injected orthotopically. The mean intensity of the bioluminescence signal emitted from the lung cancer cells increased logarithmically during the course of study. Mice with positive bioluminescence signaling had confirmed tumors by microscopic histological analysis. Bioluminescence activity had a strong correlation with the tumor volume as determined histologically. CONCLUSIONS: Bioluminescence intensity directly correlates with tumor volume and therefore offers a reliable approach for detecting and monitoring the growth of humanlung cancer cells in orthotopic murine models.
Authors: Venus Sosa Iglesias; Stefan J van Hoof; Ana Vaniqui; Lotte Ejr Schyns; Natasja Lieuwes; Ala Yaromina; Linda Spiegelberg; Arjan J Groot; Frank Verhaegen; Jan Theys; Ludwig Dubois; Marc Vooijs Journal: Br J Radiol Date: 2018-11-30 Impact factor: 3.039
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Authors: Osamu Togao; Chase W Kessinger; Gang Huang; Todd C Soesbe; Koji Sagiyama; Ivan Dimitrov; A Dean Sherry; Jinming Gao; Masaya Takahashi Journal: PLoS One Date: 2013-10-15 Impact factor: 3.240
Authors: Joseph Dragavon; Carolyn Sinow; Alexandra D Holland; Abdessalem Rekiki; Ioanna Theodorou; Chelsea Samson; Samantha Blazquez; Kelly L Rogers; Régis Tournebize; Spencer L Shorte Journal: J Vis Exp Date: 2014-05-23 Impact factor: 1.355