| Literature DB >> 20800979 |
Gaurava K Rai1, Meeta Saxena, Vidya Singh, Ramesh Somvanshi, Bhaskar Sharma.
Abstract
Papilloma viruses are detected and identified by PCR with consensus primers designed from human papilloma virus sequences. These and other primers could not detect papilloma virus in bovine teat wart samples despite repeated attempts. DNase-SISPA, a metagenomic method for identifying viruses, could identify bovine papilloma virus type 10 in bovine teat warts. The sequence comparison between consensus primers and bovine papilloma virus type 10 sequences revealed many differences between consensus primers and BPV-10 sequences. We suggest, DNase-SISPA may be used as an alternate method for papilloma virus diagnosis, in cases where PCR fails to identify papilloma viruses. Copyright ÂEntities:
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Year: 2010 PMID： 20800979 DOI： 10.1016/j.vetmic.2010.07.015
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293