BACKGROUND: The Badimon chamber is a clinical ex vivo model of thrombosis that mimics flow conditions within the coronary circulation of man. The aims of this study were to characterise thrombus formation in the chamber and evaluate its reproducibility. METHODS: Using blood from 24 healthy human volunteers, thrombus formation was assessed at low and high shear rates with porcine aortic tunica media as the thrombogenic substrate. Thrombus area was measured histomorphometrically. Reproducibility was assessed by paired measurements made both within and between days. Platelet activation was assessed before and at selected points within the extracorporeal circuit using flow cytometry, and fibrin content and distribution within the thrombus were assessed by immunohistochemistry. RESULTS: Total thrombus area was highly reproducible within and between days in the low shear ([mean thrombus area, mean difference ± SEM] 8,018μm(2), 58±204μm(2) and 8,177μm(2), -154±168μm(2) respectively) and high shear chambers (11,802μm(2), -52±175μm(2) and 11,877μm(2), 220±181μm(2) respectively). Total thrombus area was greater in the high compared to the low shear chamber (11,970±285μm(2)versus 7,892±298μm(2); P<0.0001). Transit through the extracorporeal circuit did not result in platelet activation which was only detected after blood passed across the perfusion chambers (P=0.02 for platelet-monocyte aggregate formation and P=0.05 for P-selectin expression). Thrombus in the low shear chamber contained a greater proportion of fibrin (25.0±6.0% versus 8.3±1.6%, P<0.001). CONCLUSIONS: The Badimon chamber provides a highly reproducible technique for the assessment of ex vivo platelet-rich thrombus formation in man.
BACKGROUND: The Badimon chamber is a clinical ex vivo model of thrombosis that mimics flow conditions within the coronary circulation of man. The aims of this study were to characterise thrombus formation in the chamber and evaluate its reproducibility. METHODS: Using blood from 24 healthy human volunteers, thrombus formation was assessed at low and high shear rates with porcine aortic tunica media as the thrombogenic substrate. Thrombus area was measured histomorphometrically. Reproducibility was assessed by paired measurements made both within and between days. Platelet activation was assessed before and at selected points within the extracorporeal circuit using flow cytometry, and fibrin content and distribution within the thrombus were assessed by immunohistochemistry. RESULTS: Total thrombus area was highly reproducible within and between days in the low shear ([mean thrombus area, mean difference ± SEM] 8,018μm(2), 58±204μm(2) and 8,177μm(2), -154±168μm(2) respectively) and high shear chambers (11,802μm(2), -52±175μm(2) and 11,877μm(2), 220±181μm(2) respectively). Total thrombus area was greater in the high compared to the low shear chamber (11,970±285μm(2)versus 7,892±298μm(2); P<0.0001). Transit through the extracorporeal circuit did not result in platelet activation which was only detected after blood passed across the perfusion chambers (P=0.02 for platelet-monocyte aggregate formation and P=0.05 for P-selectin expression). Thrombus in the low shear chamber contained a greater proportion of fibrin (25.0±6.0% versus 8.3±1.6%, P<0.001). CONCLUSIONS: The Badimon chamber provides a highly reproducible technique for the assessment of ex vivo platelet-rich thrombus formation in man.
Authors: Amanda L Hunter; Anoop S V Shah; Jeremy P Langrish; Jennifer B Raftis; Andrew J Lucking; Mairi Brittan; Sowmya Venkatasubramanian; Catherine L Stables; Dominik Stelzle; James Marshall; Richard Graveling; Andrew D Flapan; David E Newby; Nicholas L Mills Journal: Circulation Date: 2017-04-04 Impact factor: 29.690
Authors: Jack P M Andrews; Christophe Portal; Tashfeen Walton; Mark G Macaskill; Patrick W F Hadoke; Carlos Alcaide Corral; Christophe Lucatelli; Simon Wilson; Ian Wilson; Gillian MacNaught; Marc R Dweck; David E Newby; Adriana A S Tavares Journal: Eur Heart J Cardiovasc Imaging Date: 2020-06-01 Impact factor: 6.875
Authors: Mohammed N Meah; Jennifer Raftis; Simon J Wilson; Vidya Perera; Samira M Garonzik; Bindu Murthy; J Gerry Everlof; Ronald Aronson; Joseph Luettgen; David E Newby Journal: Arterioscler Thromb Vasc Biol Date: 2020-09-10 Impact factor: 8.311
Authors: Simon J Wilson; Thomas M Connolly; Gary Peters; Atalanta Ghosh; Maureen Johnson; David E Newby Journal: Cardiovasc Res Date: 2019-03-01 Impact factor: 10.787
Authors: Claire S Whyte; Hadj Ahmed Mostefai; Kim M Baeten; Andrew J Lucking; David E Newby; Nuala A Booth; Nicola J Mutch Journal: Int J Mol Sci Date: 2021-02-20 Impact factor: 6.208