OBJECTIVES: To determine the time-course changes of cell-free plasma DNA (cfDNA) following heavy exercise. METHODS: cfDNA concentration, C-reactive protein levels (hs-CRP), uric acid concentration (UA), creatine kinase activity (CK) were measured before and post-exercise (immediately post, 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 8h, 10h, 24h). RESULTS: cfDNA increased (15-fold) 30-min post-exercise and normalized thereafter. hs-CRP increased (56%, p<0.001) 1h post-exercise, remained elevated throughout recovery (52-142%, p<0.0001), and peaked (200% rise, p<0.0001) at 24h post-exercise. UA and CK increased (p<0.05), immediately post-exercise, remained elevated throughout recovery (p<0.0001), and peaked (p<0.0001) at 24h of post-exercise recovery. CONCLUSIONS: cfDNA sampling timing is crucial and a potential source of error following aseptic inflammation.
OBJECTIVES: To determine the time-course changes of cell-free plasma DNA (cfDNA) following heavy exercise. METHODS: cfDNA concentration, C-reactive protein levels (hs-CRP), uric acid concentration (UA), creatine kinase activity (CK) were measured before and post-exercise (immediately post, 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 8h, 10h, 24h). RESULTS: cfDNA increased (15-fold) 30-min post-exercise and normalized thereafter. hs-CRP increased (56%, p<0.001) 1h post-exercise, remained elevated throughout recovery (52-142%, p<0.0001), and peaked (200% rise, p<0.0001) at 24h post-exercise. UA and CK increased (p<0.05), immediately post-exercise, remained elevated throughout recovery (p<0.0001), and peaked (p<0.0001) at 24h of post-exercise recovery. CONCLUSIONS: cfDNA sampling timing is crucial and a potential source of error following aseptic inflammation.
Authors: Georgios Mavropalias; Leslie Calapre; Michael Morici; Tomoko Koeda; Wayne C K Poon; Oliver R Barley; Elin Gray; Anthony J Blazevich; Kazunori Nosaka Journal: Eur J Appl Physiol Date: 2021-01-13 Impact factor: 3.078
Authors: Nicholas C Eastley; Barbara Ottolini; Rita Neumann; Jin-Li Luo; Robert K Hastings; Imran Khan; David A Moore; Claire P Esler; Jacqueline A Shaw; Nicola J Royle; Robert U Ashford Journal: Oncotarget Date: 2018-01-19
Authors: Penny E Shockett; Januka Khanal; Alina Sitaula; Christopher Oglesby; William A Meachum; V Daniel Castracane; Robert R Kraemer Journal: Physiol Rep Date: 2016-01