Effects of methanol and urea on optical resolution of phenylthiohydantoin-DL-amino acids by micellar electrokinetic chromatography with sodium N-dodecanoyl-L-valinate.

Effects of methanol and urea on enantiomeric resolution of phenylthiohydantoin (PTH)-DL-amino acids by micellar electrokinetic chromatography with sodium N-dodecanoyl-L-valinate (SDVal) were investigated. PTH-DL-Met that could not be optically resolved with an SDVal solution alone was resolved by using an SDVal/methanol solution. Four PTH-DL-amino acids were separated from each other and each pair of enantiomers was also optically resolved, although seriously tailed peaks were observed for each solute. The addition of urea to SDVal/methanol solutions improved these peak shapes.