CONTEXT: Allanblackia floribunda Oliver (Guttiferae) is an African medicinal plant used traditionally to treat a variety of ailments. OBJECTIVE: We investigated the antitumor, radical scavenging, antimycobacterial, antibacterial and antifungal activities of the root bark extract of A. floribunda and three isolated phenolics, namely 1,7-dihydroxyxanthone (1), morelloflavone (2) and 7'-O-glucoside of morelloflavone (3). MATERIALS AND METHODS: The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay was used for antioxidant tests, while crown gall tumor assay was used for assay of antitumor activity. The p-iodonitrotetrazolium chloride (INT) colorimetry and Microplate Alamar Blue Assay (MABA) were used for antimicrobial investigations. RESULTS: Moderate tumor reducing activity was observed with the extract, while better activities were recorded with compounds 2 and 3. The antimycobacterial and antitumor activities of the extract are being reported for the first time. The DPPH radical scavenging test showed that all the studied samples were able to scavenge more than 50% of the free radical, with compound 3 showing the best inhibitory activity (IC(50) of 49.08 µg/mL). Compounds 1 to 3 prevented the growth of Mycobacterium smegmatis and both extract and compound 2 were active on M. tuberculosis. The lowest MIC value for the extract (9.76 μg/mL) was recorded against Enterobacter aerogenes while the corresponding value for the compounds (4.88 µg/mL) was obtained with compound 2 on Trichophyton rubrum. DISCUSSION AND CONCLUSION: The overall results of the present work provide baseline information for the potential use of the root bark extract of A. floribunda as an antimicrobial, antitumor and antioxidant phytomedicine.
CONTEXT: Allanblackia floribunda Oliver (Guttiferae) is an African medicinal plant used traditionally to treat a variety of ailments. OBJECTIVE: We investigated the antitumor, radical scavenging, antimycobacterial, antibacterial and antifungal activities of the root bark extract of A. floribunda and three isolated phenolics, namely 1,7-dihydroxyxanthone (1), morelloflavone (2) and 7'-O-glucoside of morelloflavone (3). MATERIALS AND METHODS: The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay was used for antioxidant tests, while crown gall tumor assay was used for assay of antitumor activity. The p-iodonitrotetrazolium chloride (INT) colorimetry and Microplate Alamar Blue Assay (MABA) were used for antimicrobial investigations. RESULTS: Moderate tumor reducing activity was observed with the extract, while better activities were recorded with compounds 2 and 3. The antimycobacterial and antitumor activities of the extract are being reported for the first time. The DPPH radical scavenging test showed that all the studied samples were able to scavenge more than 50% of the free radical, with compound 3 showing the best inhibitory activity (IC(50) of 49.08 µg/mL). Compounds 1 to 3 prevented the growth of Mycobacterium smegmatis and both extract and compound 2 were active on M. tuberculosis. The lowest MIC value for the extract (9.76 μg/mL) was recorded against Enterobacter aerogenes while the corresponding value for the compounds (4.88 µg/mL) was obtained with compound 2 on Trichophyton rubrum. DISCUSSION AND CONCLUSION: The overall results of the present work provide baseline information for the potential use of the root bark extract of A. floribunda as an antimicrobial, antitumor and antioxidant phytomedicine.
Authors: Francis Ackah Armah; Isaac Tabiri Henneh; John Alake; Wisdom Ahlidja; Benjamin Amoani; Eric Gyamerah Ofori; Baffour Asante-Kyei; Gbadamosi Ismail Temitayo; Christian Kweku Adokoh Journal: Biomed Res Int Date: 2021-01-11 Impact factor: 3.411