Daniel Weiss1, Mario Koopmann, Claudia Rudack. 1. Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Muenster, Muenster, Germany. daniel.weiss@ukmuenster.de
Abstract
BACKGROUND: Human papillomavirus (HPV) is a basic risk factor for head and neck squamous cell carcinoma (HNSCC). Little knowledge exists about the impact of HPV on clinical diagnostic and therapy of patients with HNSCC. METHODS: We evaluated the evidence of HPV16 in 131 retrospectively collected HNSCC and associated cervical lymph node metastases by HPV16 real-time polymerase chain reaction (PCR) and p16 immunohistochemistry and its impact on clinicopathological characteristics. RESULTS: HPV16-DNA and p16 overexpression were present in 27% of HNSCCs. All cervical lymph node metastases of HPV16-positive HNSCC showed HPV16-DNA. HPV16 was strongly associated with tumors arising from the oropharyngeal site (p < .000001), favorable outcome after standard therapy in univariate (p = .001) and multivariate (p = .0004) analysis, and cervical lymph node metastases before primary detection. CONCLUSION: HPV16-diagnostic in cervical lymph node metastases can predict the site of tumor origin in case of carcinoma of unknown primary (CUP) and favorable outcome and should, therefore, be included in routine diagnostic workup.
BACKGROUND:Human papillomavirus (HPV) is a basic risk factor for head and neck squamous cell carcinoma (HNSCC). Little knowledge exists about the impact of HPV on clinical diagnostic and therapy of patients with HNSCC. METHODS: We evaluated the evidence of HPV16 in 131 retrospectively collected HNSCC and associated cervical lymph node metastases by HPV16 real-time polymerase chain reaction (PCR) and p16 immunohistochemistry and its impact on clinicopathological characteristics. RESULTS:HPV16-DNA and p16 overexpression were present in 27% of HNSCCs. All cervical lymph node metastases of HPV16-positive HNSCC showed HPV16-DNA. HPV16 was strongly associated with tumors arising from the oropharyngeal site (p < .000001), favorable outcome after standard therapy in univariate (p = .001) and multivariate (p = .0004) analysis, and cervical lymph node metastases before primary detection. CONCLUSION:HPV16-diagnostic in cervical lymph node metastases can predict the site of tumor origin in case of carcinoma of unknown primary (CUP) and favorable outcome and should, therefore, be included in routine diagnostic workup.
Authors: Justin A Bishop; Zahra Maleki; Alexandra Valsamakis; Takenori Ogawa; Xiaofei Chang; Sara I Pai; William H Westra Journal: Cancer Cytopathol Date: 2011-07-12 Impact factor: 5.284
Authors: Jos Straetmans; Julia Vent; Martin Lacko; Ernst-Jan Speel; Christian Huebbers; Robert Semrau; Frank Hoebers; Zlatan Mujagic; Jens-Peter Klussmann; Simon F Preuss; Bernd Kremer Journal: Eur Arch Otorhinolaryngol Date: 2014-03-11 Impact factor: 2.503
Authors: Paweł Golusiński; Jakub Pazdrowski; Mateusz Szewczyk; Maciej Misiołek; Wioletta Pietruszewska; Janusz Klatka; Sławomir Okła; Henryk Kaźmierczak; Andrzej Marszałek; Violetta Filas; Augusto Schneider; Michał M Masternak; Katarzyna Stęplewska; Katarzyna Miśkiewicz-Orczyk; Wojciech Golusiński Journal: Rep Pract Oncol Radiother Date: 2017-04-22
Authors: Silke Wemmert; Maximilian Linxweiler; Cornelia Lerner; Florian Bochen; Philipp Kulas; Johannes Linxweiler; Sigrun Smola; Steffi Urbschat; Stefan Wagenpfeil; Bernhard Schick Journal: J Cancer Res Clin Oncol Date: 2018-03-20 Impact factor: 4.553
Authors: Annekatrin Coordes; Klaus Lenz; Xu Qian; Minoo Lenarz; Andreas M Kaufmann; Andreas E Albers Journal: Eur Arch Otorhinolaryngol Date: 2015-07-31 Impact factor: 2.503
Authors: Jennifer L Hunt; Leon Barnes; James S Lewis; Magdy E Mahfouz; Pieter J Slootweg; Lester D R Thompson; Antonio Cardesa; Kenneth O Devaney; Douglas R Gnepp; William H Westra; Juan P Rodrigo; Julia A Woolgar; Alessandra Rinaldo; Asterios Triantafyllou; Robert P Takes; Alfio Ferlito Journal: Eur Arch Otorhinolaryngol Date: 2013-03-07 Impact factor: 2.503