PURPOSE: create a 3-Dimensional artificial human ovary to mature human oocytes. METHODS: theca and granulosa cells were isolated from antral follicles of reproductive-aged women, seeded into micro-molded gels and self-assembled into complex 3D microtissues. Immunohistochemistry and live-dead staining confirmed theca cell identity and cellular viability at one week respectively. Placement of granulosa cell spheroids or cumulus-oocyte complexes into theca cell honeycomb openings resulted in creation of an artificial human ovary. Oocytes from this construct were assessed for polar body extrusion. RESULTS: theca and granulosa cells self-assembled into complex microtissues, remaining viable for one week. At 72 h after artificial human ovary construction, theca cells completely surrounded the granulosa spheroids or COCs without stromal invasion or disruption. Polar body extrusion occurred in one of three COCs assessed. CONCLUSIONS: an artifical human ovary can be created with self-assembled human theca and granulosa cell microtissues, and used for IVM and future oocyte toxicology studies.
PURPOSE: create a 3-Dimensional artificial human ovary to mature human oocytes. METHODS: theca and granulosa cells were isolated from antral follicles of reproductive-aged women, seeded into micro-molded gels and self-assembled into complex 3D microtissues. Immunohistochemistry and live-dead staining confirmed theca cell identity and cellular viability at one week respectively. Placement of granulosa cell spheroids or cumulus-oocyte complexes into theca cell honeycomb openings resulted in creation of an artificial human ovary. Oocytes from this construct were assessed for polar body extrusion. RESULTS: theca and granulosa cells self-assembled into complex microtissues, remaining viable for one week. At 72 h after artificial human ovary construction, theca cells completely surrounded the granulosa spheroids or COCs without stromal invasion or disruption. Polar body extrusion occurred in one of three COCs assessed. CONCLUSIONS: an artifical human ovary can be created with self-assembled human theca and granulosa cell microtissues, and used for IVM and future oocyte toxicology studies.
Authors: Che-Ying Kuo; Hannah Baker; Melissa H Fries; James J Yoo; Peter C W Kim; John P Fisher Journal: Tissue Eng Part B Rev Date: 2017-02-03 Impact factor: 6.389
Authors: D Durand-Herrera; F Campos; B D Jaimes-Parra; J D Sánchez-López; R Fernández-Valadés; M Alaminos; A Campos; V Carriel Journal: Histochem Cell Biol Date: 2018-06-11 Impact factor: 4.304