Literature DB >> 20734277

Immunocytological analysis of chromatin in isolated nuclei.

Penka Pavlova1, Federico Tessadori, Hans J de Jong, Paul Fransz.   

Abstract

All cells in a multicellular organism have the same genetic constitution, yet their appearance and function may differ enormously, due to differences in the nuclear program. Central in the establishment of this cell diversity are epigenetic marks, which are largely based on covalent modifications of histones and methylated cytosine residues in the DNA sequence. The study of these epigenetic factors in individual cells requires the microscopic visualization of chromatin components. Here we describe a number of protocols to study chromatin in isolated nuclei.

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Year:  2010        PMID： 20734277     DOI： 10.1007/978-1-60761-765-5_28

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Seed maturation in Arabidopsis thaliana is characterized by nuclear size reduction and increased chromatin condensation.

Authors:  Martijn van Zanten; Maria A Koini; Regina Geyer; Yongxiu Liu; Vittoria Brambilla; Dorothea Bartels; Maarten Koornneef; Paul Fransz; Wim J J Soppe
Journal:  Proc Natl Acad Sci U S A       Date:  2011-11-28       Impact factor: 11.205

2.  Control and consequences of chromatin compaction during seed maturation in Arabidopsis thaliana.

Authors:  Martijn van Zanten; Annaick Carles; Yong Li; Wim J J Soppe
Journal:  Plant Signal Behav       Date:  2012-03-01

3.  Genetic Dissection of Morphometric Traits Reveals That Phytochrome B Affects Nucleus Size and Heterochromatin Organization in Arabidopsis thaliana.

Authors:  Basten L Snoek; Penka Pavlova; Federico Tessadori; Anton J M Peeters; Clara Bourbousse; Fredy Barneche; Hans de Jong; Paul F Fransz; Martijn van Zanten
Journal:  G3 (Bethesda)       Date:  2017-08-07       Impact factor: 3.154

4.  Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis.

Authors:  Kinga Rutowicz; Maciej Lirski; Benoît Mermaz; Gianluca Teano; Jasmin Schubert; Imen Mestiri; Magdalena A Kroteń; Tohnyui Ndinyanka Fabrice; Simon Fritz; Stefan Grob; Christoph Ringli; Lusik Cherkezyan; Fredy Barneche; Andrzej Jerzmanowski; Célia Baroux
Journal:  Genome Biol       Date:  2019-08-07       Impact factor: 13.583

5.  2D morphometric analysis of Arabidopsis thaliana nuclei reveals characteristic profiles of different cell types and accessions.

Authors:  Penka Pavlova; Martijn van Zanten; Basten L Snoek; Hans de Jong; Paul Fransz
Journal:  Chromosome Res       Date:  2021-10-19       Impact factor: 5.239

6.  Arabidopsis shoot stem cells display dynamic transcription and DNA methylation patterns.

Authors:  Ruben Gutzat; Klaus Rembart; Thomas Nussbaumer; Falko Hofmann; Rahul Pisupati; Gabriele Bradamante; Nina Daubel; Angelika Gaidora; Nicole Lettner; Mattia Donà; Magnus Nordborg; Michael Nodine; Ortrun Mittelsten Scheid
Journal:  EMBO J       Date:  2020-08-20       Impact factor: 14.012
  6 in total

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